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  • 1.
    Berggren, Elisabeth
    et al.
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för omvårdnad. Högskolan i Jönköping, Hälsohögskolan, HHJ. Kvalitetsförbättringar, innovationer och ledarskap inom vård och socialt arbete.
    Sidenvall, Birgitta
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för omvårdnad.
    Larsson, Dennis
    Memory ability after subarachnoid haemorrhage: Relatives´and patients´statements in relation to test results2010Inngår i: British Journal of Neuroscience Nursing, ISSN 1747-0307, E-ISSN 2052-2800, Vol. 6, nr 8, s. 383-391Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aim: The aim of this study was to describe memory after a subarachnoid haemorrhage (SAH) from the perspective of relatives and patients in two cohorts and also to evaluate the application of relatives' statements as a tool in nursing care and rehabilitation, in order to support the patient. Background: Cognitive sequelae due to SAH are a large disability and may influence the adjustment to daily life. Supporting patients and relatives requires knowledge concerning the patients' memory both from the perspective of patients and relatives. Method: Eleven relatives and 11 patients (Cohort 1), 11 years after the onset of an SAH and 15 relatives and 15 patients (Cohort 2) 6 years after the onset of an SAH, participated in the study. Interview questions and memory tests were used to collect data. Findings: Problems with memory, including meta-memory problems regarding relatives' statements, were common. Relatives and patients stated patients' memory in a similar manner. However, patients' statements concerning their memory corresponded in higher degree with memory test results, in comparison with relatives' statements. Conclusions: Relatives' and patients' statements are useful as tools in nursing care and rehabilitation. However, from results showing meta-memory problems and that patients' statements concerning their memory corresponded better with memory test results (in comparison with relatives' statements), it is vital to offer patients memory tests in order to prevent complications in mutual family relationships.

  • 2.
    Berggren, Elisabeth
    et al.
    Högskolan i Jönköping, Hälsohögskolan, HHJ. Kvalitetsförbättringar, innovationer och ledarskap inom vård och socialt arbete.
    Sidenvall, Birgitta
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Subarachnoid haemorrhage has long-term effects on social life2011Inngår i: British Journal of Neuroscience Nursing, ISSN 1747-0307, E-ISSN 2052-2800, Vol. 7, nr 1, s. 429-435Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aim: The aim of this study was to describe activities of living after a subarachnoid haemorrhage (SAH) from the perspective of relatives and patients in two cohorts and to evaluate the application of relatives’ statements, as a tool in nursing care, in order to support the patient.

    Background: Memory problems after SAH are common according to patients’ and relatives’ statements and memory test results. This may influence the adjustment to daily life. Supporting patients and relatives requires knowledge concerning the activities of daily living from the perspective of both patients and relatives.

    Method:Eleven relatives and 11 patients (cohort 1), 11 years after the onset of an SAH and 15 relatives and 15 patients (cohort 2) 6 years after the onset of an SAH participated in the study. Interview questions and memory tests were used to collect data.

    Findings:Problems with activities of daily living were common and patients had more problems with social life than with personal and instrumental activites. The change in social company habits were due to emotional problems. Patients’ statements about problems with activities of living corresponded to results from patients’ memory tests and patients’ statements.

    Conclusions: Relatives’ and patients’ statements are useful as tools in nursing care. Introducing fromalized dialogues and memory tests would improve the after care for this group of patients and improve their future family relationships.

  • 3.
    Holmén, Jonathan
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Jansson, Andreas
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    A Kinetic Overview of the Receptors Involved in 1,25-Dihydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 Signaling: A Systems Biology Approach2009Inngår i: Critical Reviews in Eukaryotic Gene Expression, ISSN 1045-4403, E-ISSN 2162-6502, Vol. 19, nr 3, s. 181-196Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    The vitamin D endocrine system modulates an arsenal of important biological functions in more than 30 different tissues in short- and long-term perspectives. Two membrane receptors and one nuclear receptor are suggested to be involved in the vitamin D signaling system, but the function and physiological relevance of the receptors are debated. The complexity of the vitamin D endocrine system makes it necessary to combine experimental data with in silico simulations to get a holistic view of vitamin D-dependent regulation of tissue and cell physiology. This review focus on binding characteristics for the three putative vitamin D receptors and proposes a future systems biology approach including mathematical modeling that will be helpful together with experimental methods in depicting antitumoral and other biological effects promoted by the vitamin D endocrine system.

  • 4.
    Karlsson, Sandra
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Olausson, Josefin
    Högskolan i Skövde, Institutionen för vård och natur.
    Lundh, Dan
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sögård, Peter
    Högskolan i Skövde, Institutionen för vård och natur.
    Mandal, Abul
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Holmström, Kjell-Ove
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Stahel, Anette
    Högskolan i Skövde, Institutionen för vård och natur.
    Bengtsson, Jenny
    Högskolan i Skövde, Institutionen för vård och natur.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Vitamin D and prostate cancer: The role of membrane initiated signaling pathways in prostate cancer progression2010Inngår i: Journal of Steroid Biochemistry and Molecular Biology, ISSN 0960-0760, E-ISSN 1879-1220, Vol. 121, nr 1-2, s. 413-416Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been demonstrated to mediate both genomic and non-genomic responses in prostate cancer (CaP) cells. Here, we give an overview of membrane initiated 1,25(OH)2D3 signaling in prostate cancer cell progression. The presence of PDIA3 was investigated and homologous modeling of the putative PDIA3 receptor complex was conducted. Furthermore, the cellular distribution of nVDR was analyzed. We could show that both nVDR and PDIA3 are expressed in the prostate cancer cell lines investigated. The homologous modeling of PDIA3 showed that the receptor complex exists in a trimer formation, which suggests for allosteric activity. Our findings support previous reports and suggest that 1,25(OH)2D3 is an important therapeutic agent in inhibiting prostate cancer progression. Furthermore, our data show that 1,25(OH)2D3 regulate prostate cell biology via multiple pathways and targeting specific pathways for 1,25(OH)2D3 might provide more effective therapies compared to the vitamin D therapies currently clinically tested.

  • 5.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Anderson, Deryk
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States / Kansas City University of Medicine and Biosciences, Kansas City, MO, United States.
    Smith, Nathan M.
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States.
    Nemere, Ilka
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States / Department of Nutrition and Food Sciences, Utah State University, Logan, UT, United States.
    24,25-Dihydroxyvitamin D3 binds to catalase2006Inngår i: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 97, nr 6, s. 1259-1266Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    There is increasing evidence that the vitamin D metabolite, 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) has endocrine actions. In the current work, we report that an endogenous binding protein for 24,25(OH)2D3 is catalase, based on sequence analysis of the isolated protein. An antibody (Ab 365) generated against equivalent protein recognized bovine catalase and a 64 kDa band in subcellular fractions of chick intestine. A commercially available anti-catalase antibody reduced specific [3H]24,25(OH)2D3 binding in subcellular fractions of chick intestine by greater than 65%, relative to the same fractions treated with an unrelated antibody (Ab 099). The same commercially available anti-catalase was able to block the inhibitory actions of 24,25(OH)2D3 on 32P uptake in isolated intestinal epithelial cell suspensions. We subsequently characterized binding of steroid to commercially available catalase, and found that between 0 and 5 nM of enzyme added to subcellular fraction P2 (20,000g, 10-min post-nuclear pellet) resulted in a linear increase in the amount of [3H]24,25(OH)2D3 specifically bound. Additional studies indicated that 25(OH)D3 was an effective competitor for binding, whereas 1,25(OH)2D3 only poorly displaced [3H]24,25(OH)2D3. Saturation analyses with added catalase yielded a physiologically relevant affinity constant (KD = 5.6 ± 2.7 nM) and a Bmax = 209 ± 34 fmols/mg protein, comparable to previous studies using purified basal lateral membranes or vesicular fractions. Moreover, in a study on subcellular fractions isolated from chickens of varying ages, we found that in females, both specific [3H]24,25(OH)2D3 binding and catalase activity increased from 7- to 58-week-old birds, whereas in males, elevated levels of both parameters were expressed in preparations of 7- and 58-week-old birds. The data suggest that signal transduction may occur through modulation of hydrogen peroxide production.

  • 6.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Hagberg, Malin
    Högskolan i Skövde, Institutionen för vård och natur.
    Nahren, Malek
    Högskolan i Skövde, Institutionen för vård och natur.
    Kjellberg, Charlotte
    Högskolan i Skövde, Institutionen för vård och natur.
    Senneberg, Edina
    Högskolan i Skövde, Institutionen för vård och natur.
    Tahmasebifar, Neda
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson, Viktoria
    Karolinska Institutet.
    Membrane Initiated Signaling by 1,25α-dihydroxyvitamin D3 in LNCaP Prostate Cancer Cells2008Inngår i: Hormonal Carcinogenesis V / [ed] Jonathan J. Li, Sara A. Li, Suresh Mohla, Henri Rochefort, Thierry Maudelonde, Springer, 2008, s. 573-579Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    Prostate cancer (PC) is one of the most common cancers among men, and vitamin D and its metabolites are candidates for prevention and therapy of this disease. The vitamin D metabolites, 1, 25-dihydroxyvitamin D3 (1,25D) and 25-hydroxyvitamin D3, decreases cellular proliferation and invasiveness, and stimulates differentiation of PC cells. However, the underlying mechanisms are not fully clarified, and there is evidence that some of these effects of the vitamin D system are mediated by specific membrane-associated receptors/binding proteins in addition to its nuclear receptor, suggesting multiple regulatory pathways. The aim of the present study was to examine the role of membrane initiated pathways mediating effects of 1,25D on cell invasiveness in LNCaP cells. Treatment with 1,25D evoked a dose-dependent activation of the JNK/SAPK MAPK signaling pathways within 10 min, demonstrating membrane initiated signaling of 1,25D in LNCaP cells. Furthermore, treatment with 1,25D decreased LNCaP cell invasiveness by approximately 20% after 48h. Using an inhibitor (SP600125) for the JNK/SAPK MAPK signaling pathway in combination with 1,25D on LNCaP cells, the inhibitory action of 1,25D on invasiveness was eliminated. In conclusion, 1,25D decrease invasiveness of LNCaP cells by interaction with a putative membrane associated receptor, which activate membrane, initiated signaling via the JNK/SAPK MAPK signaling pathway.

  • 7.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson, Viktoria
    Högskolan i Skövde, Institutionen för vård och natur.
    Karlsson, Sandra
    Högskolan i Skövde, Institutionen för vård och natur.
    Olausson, Josefin
    Högskolan i Skövde, Institutionen för vård och natur.
    Holmén, Jonathan
    Högskolan i Skövde, Institutionen för vård och natur.
    Hagberg, Malin
    Högskolan i Skövde, Institutionen för vård och natur.
    Rapid activation of JNK/SAPK in LNCaP prostate cancer cells by 1α,25-dihydroxyvitamin D3 is independent of PDIA3 (1,25-MARRS)2008Inngår i: Current Topics in Steroid Research, ISSN 0972-4788, Vol. 5, s. 17-24Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    1α,25-dihydroxyvitamin D3 (1,25D3 ) is a highly potential anti-cancerous agent for prevention and treatment of prostate cancer, the most commonly diagnosed cancer type of males in western countries. A recent study by our laboratory, demonstrates that LNCaP cancer cells treated with 1,25D3, evoked dose-dependent activation of the JNK/SAPK MAPK signaling pathway within 10 minutes after hormone treatment, indicative of membrane-initiated steroid signaling (MISS) by 1,25D3. This confirms previous reports on intestinal-, chondrocyte- and osteoblast cells, where 1,25D3 operates through pharmacologically distinct nuclear-initiated mechanisms (NISS) and plasma membrane-initiated mechanisms. NISS is mediated via the vitamin D receptor (nVDR) and MISS is mediated through 1,25D3-MARRS (PDIA3, 1,25D3-membraneassociated rapid response steroid binding protein) or nVDR. The aims of the present study were to investigate the mechanisms of MISS evoked effects on alkaline phosphatase (ALP) and activation of the JNK/SAPK by 1,25D3, and the involvement of PDIA3 in 1,25D3 initiated activation of the JNK/SAPK signaling pathway. Furthermore, 1,25D3-treated LNCaP cells were transfected with siRNA against PDIA3 and phosphorylated JNK/SAPK was estimated by western analysis. Western analysis and ALP-assays demonstrated rapid activation of both JNK/SAPK as well as ALP. Silencing of PDIA3 did not affect 1,25D3 mediated activation of JNK/SAPK, suggesting that PDIA3 is not involved in the 1,25D3-initiated activation of the JNK/SAPK signaling pathway.

  • 8.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Jonas, Adele
    Högskolan i Skövde, Institutionen för vård och natur.
    Bergsten, Niklas
    Högskolan i Skövde, Institutionen för vård och natur.
    Ståhl, Fredrik
    University College of Borås, School of Health Sciences, Sweden.
    Karlsson, Sandra
    Högskolan i Skövde, Institutionen för vård och natur.
    Membrane Initiated Effects of1α,25-Dihydroxyvitamin D3 inProstate Cancer Cells: Effects on AP1 and CREB Mediated Transcription2012Inngår i: Current Frontiers and Perspectives in Cell Biology / [ed] Stevo Najman, InTech, 2012, s. 153-162Kapittel i bok, del av antologi (Fagfellevurdert)
  • 9.
    Lundh, Dan
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Hedelin, Hans
    Skaraborgs Sjukhus, Skövde , Sweden.
    Jonsson, Karin
    Kärnsjukhuset, Skövde , Sweden.
    Gifford, Mervyn
    Högskolan i Skövde, Institutionen för vård och natur.
    Larsson, Dennis
    Högskolan i Skövde, Institutionen för vård och natur.
    Assessing chronic pelvic pain syndrome patients: Blood plasma factors and cortisol saliva2013Inngår i: Scandinavian journal of urology, ISSN 2168-1805, E-ISSN 2168-1813, Vol. 47, nr 6, s. 521-528Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective:

    The aim of this study was to identify changes in inflammatory molecules in the blood (plasma) of patients with chronic prostatitis/chronic pelvic syndrome (CP/CPPS) compared with controls. Altered levels indicate a systemic component by possible involvement of the prostate and/or the inner pelvic floor musculature.

    Material and methods:

    In 32 patients with CP/CPPS and 37 controls, blood plasma levels of testosterone, macrophage migration inhibitory factor (MIF), tumour necrosis factor-alpha (TNF-alpha), TNF-beta, interleukin-2 (IL-2) and IL-1 beta were measured by enzyme-linked immunosorbent assay. Cortisol in saliva samples was measured in the morning and late evening. All participants answered a questionnaire regarding their health profile.

    Results:

    Significantly higher levels of MIF (p = 0.012) were detected in patients. The testosterone level was, contrary to other studies, little lower in patients (p = 0.014; age adjusted). When controls with health issues and patients with a parallel disease were excluded, the MIF and TNF-alpha levels were higher in the patients (p = 0.007, p = 0.016, respectively) than in controls, and the testosterone was slightly lower in patients (p = 0.047).

    Conclusions:

    The findings show an immune response extending to the circulatory system, in which MIF makes a significant contribution to CP/CPPS. This study also indicates TNF-alpha as a circulatory component when excluding subjects with concomitant diseases. Both MIF and TNF-alpha have previously been highlighted for other diseases related to chronic pain and here also for CP/CPPS. These results provide further insights into the immunological basis of CP/CPPS.

  • 10.
    Lundh, Dan
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Hedelin, Hans
    Department of Research and Development, Skaraborgs Sjukhus, Skövde, Sweden.
    Larsson, Dennis
    Högskolan i Skövde, Institutionen för vård och natur.
    Chronic prostatitis/chronic pelvic pain syndrome: Interplay of inflammatory mediators, "Beyond the Abstract"2013Annet (Annet vitenskapelig)
  • 11.
    Lundh, Dan
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Nahar, Noor
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Mandal, Abul
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Arsenic accumulation in plants - Outlining strategies for developing improved variety of crops for avoiding arsenic toxicity in foods2010Inngår i: Journal of biological systems, ISSN 0218-3390, Vol. 18, nr 1, s. 223-241Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Contamination of food with arsenics is a potential health risk for both humans and animals in many regions of the world, especially in Asia. Arsenics can be accumulated in humans, animals and plants for a longer period and a long-term exposure of humans to arsenics results in severe damage of kidney, lever, heart etc. and many other vascular diseases. Arsenic contamination in human may also lead to development of cancer. In this paper we report our results on data mining approach (an in silico analysis based on searching of the existing genomic databases) for identification and characterization of genes that might be responsible for uptake, accumulation or metabolism of arsenics. For these in silico analyses we have involved the model plant Arabidopsis thaliana in our investigation. By employing a system biology model (a kinetic model) we have studied the molecular mechanisms of these processes in this plant. This model contains equations for uptake, metabolism and sequestration of different types of arsenic; As(V), As(III), MMAA and DMAA. The model was then implemented in the software XPP. The model was also validated against the data existing in the literatures. Based on the results of these in silico studies we have developed some strategies that can be used for reducing arsenic contents in different parts of the plant. Data mining experiments resulted in identification of two candidate genes (ACR2, arsenate reductase 2 and PCS1, phytochelatin synthase 1) that are involved either in uptake, transport or cellular localization of arsenic in A. thaliana. However, our system biology model revealed that by increasing the level of arsenate reductase together with an increased rate of arsenite sequestration in the vacuoles (by involving an arsenite efflux pump MRP1/2), it is possible to reduce the amount of arsenics in the shoots of A. thaliana to 11–12%.

  • 12.
    Sogaard, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Szekeres, Ferenc
    Karolinska Institutet.
    Garcia-Roves, Pablo M.
    Karolinska Institutet.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Chibalin, Alexander V.
    Karolinska Institutet.
    Zierath, Juleen R.
    Karolinska Institutet.
    Spatial Insulin Signalling in Isolated Skeletal Muscle Preparations2010Inngår i: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 109, nr 5, s. 943-949Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    During in vitro incubation in the absence or presence of insulin, glycogen depletion occurs in the inner core of the muscle specimen, concomitant with increased staining of hypoxia-induced-factor-1-alpha and caspase-3, markers of hypoxia and apoptosis, respectively. The aim of this study was to determine whether insulin is able to diffuse across the entire muscle specimen in sufficient amounts to activate signalling cascades to promote glucose uptake and glycogenesis within isolated mouse skeletal muscle. Phosphoprotein multiplex assay on lysates from muscle preparation was performed to detect phosphorylation of insulin-receptor on Tyr1146, Akt on Ser473 and glycogen-synthases-kinase-3 on Ser21/Ser9. To address the spatial resolution of insulin signalling, immunohistochemistry studies on cryosections were performed. Our results provide evidence to suggest that during the in vitro incubation, insulin sufficiently diffuses into the centre of tubular mouse muscles to promote phosphorylation of these signalling events. Interestingly, increased insulin signalling was observed in the core of the incubated muscle specimens, correlating with the location of oxidative fibres. In conclusion, insulin action was not restricted due to insufficient diffusion of the hormone during in vitro incubation in either extensor digitorum longus or soleus muscles from mouse under the specific experimental settings employed in this study. Hence, we suggest that the glycogen depleted core as earlier observed is not due to insufficient insulin action.

  • 13.
    Sundh, Henrik
    et al.
    Fish Endocrinology Laboratory, Department of Zoology, Göteborg University, Box 463, SE-405 30 Göteborg, Sweden.
    Larsson, Dennis
    Högskolan i Skövde, Institutionen för vård och natur.
    Sundell, Kristina
    Fish Endocrinology Laboratory, Department of Zoology, Göteborg University, Box 463, SE-405 30 Göteborg, Sweden.
    Environmental salinity regulates the in vitro production of [3H]-1,25-dihydroxyvitamin D3 and [3H]-24,25 dihydroxyvitamin D3 in rainbow trout (Oncorhynchus mykiss)2007Inngår i: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 152, nr 2-3, s. 252-258Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Previous studies have shown that specific binding of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) to enterocyte basolateral membranes (BLM), as well as circulating concentrations, is affected in response to changes in environmental salinity. It is not known if the production of 1,25(OH)2D3 and 24,25(OH)2D3 is affected by environmental salinity. The aim of the present study was to measure the in vitro production of [3H]-1,25(OH)2D3 and [3H]-24,25(OH)2D3 in fresh water (FW) and after 1, 2, 3, and 7 days after transfer to seawater (SW). Pooled sub-cellular fractions (mitochondria and microsomes) from liver or kidney was incubated with [3H]-25(OH)D3 and the produced metabolites were separated using HPLC. Hepatic production of [3H]-1,25(OH)2D3 was decreased after 24 h in SW. This was followed by an up-regulation after 48 h and a second, slower decrease in production rate which leveled out after 7 days in SW. The production rate in SW was lower than the original rate in FW-adapted fish. For hepatic [3H]-24,25(OH)2D3 production the pattern was reversed. Renal production of [3H]-24,25(OH)2D3 increased significantly during the period of SW acclimation. These results suggest that environmental salinity regulates the production rate of the two antagonizing calcium regulatory hormones; 1,25(OH)2D3 and 24,25(OH)2D3. This gives further evidence to the hypothesis that there is a physiological regulation and a differentiated importance of 1,25(OH)2D3 and 24,25(OH)2D3 in relation to environmental calcium concentrations.

  • 14.
    Sögaard, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Szekeres, Ferenc
    Department of Molecular Medicine and Surgery, Section og Integrative Physiology, Karolinska Institutet.
    Holmström, Maria
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Harlén, Mikael
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Garcia-Roves, Pablo
    Section of Integrative Physiology, Department of Physiology and Pharmacology, Karolinska Institutet.
    Chibalin, Alexander V.
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Effects of fibre type and diffusion distance on mouse skeletal muscle glycogen content in vitro2009Inngår i: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 107, nr 6, s. 1189-1197Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In vitro incubation of isolated rodent skeletal muscle is a widely used procedure in metabolic research. One concern with this method is the development of an anoxic state during the incubation period that can cause muscle glycogen depletion. Our aim was to investigate whether in vitro incubation conditions influence glycogen concentration in glycolytic extensor digitorum longus (EDL) and oxidative soleus mouse muscle. Quantitative immunohistochemistry was applied to assess glycogen content in incubated skeletal muscle. Glycogen concentration was depleted, independent of insulin-stimulation in the incubated skeletal muscle. The extent of glycogen depletion was correlated with the oxidative fibre distribution and with the induction of hypoxia-induced-factor-1-alpha. Insulin exposure partially prevented glycogen depletion in soleus, but not in EDL muscle, providing evidence that glucose diffusion is not a limiting step to maintain glycogen content. Our results provide evidence to suggest that the anoxic milieu and the intrinsic characteristics of the skeletal muscle fibre type play a major role in inducing glycogen depletion in during in vitro incubations.

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