Safe bacterial diagnostics requires proper sampling techniques, suitable specimen transport and correct inoculation techniques. As a result of centralization of operations and economic restraints, transportation of samples to microbiology laboratories is very common. This entails the need for a transport medium that keeps sensitive bacteria alive under the long transport and storage time. Additionally, the transport tube should be convenient for automatic inoculation that requires a liquid based medium. This transition also reduces workload and improves ergonomics of the staff. In this study the survival of Propionibacterium acnes (n = 3), Bacteroides fragilis (n = 2) and Neisseria gonorrheae (n = 3) in the transport medium Copan E-swab™ was evaluated. All the species were inoculated in the tubes for 24, 48, 72h and also 120h for B. fragilis and P. acnes. Initial concentration at start time and the percentage survival over time was calculated with the help of viable count. Viability decreased to 47 – 80 % for P. acnes, 18 % for B. fragilis 1 and 73% for B. fragilis 2 after 120 h storage in the transportmedium. N. gonorrhoeae showed a reduction in viability of 96 – 99,97 % after 24h storage in the medium at 4°C and in room temperature. P. acnes and B. fragilis can be stored in the medium for up to 5 days, without endangering a diagnosis. N. gonorrhoeae can be stored in the transport medium for up to 24h to secure a diagnosis through culturing.