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  • 1.
    Johansson, Linnéa
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
    Isaksson, Malin
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
    Prevalens av Trichomonas vaginalis i STI-prover från Västra Götalands län med Aptima™ TV Assay på Panther™ System: Samt utvärdering av Aptima™ TV Assay på Panther™ System med jämförelse mot Xpert® TV Kit på GeneXpert®2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Abstract

    Prevalence of Trichomonas vaginalis in STI-samples from Västra Götaland county with Aptima™ TV Assay on Panther™ System

    Also, evaluation of Aptima™ TV Assay on Panther™ System with comparison with Xpert® TV Kit on GeneXpert®

    Trichomonas vaginalis is a parasite that is spread by sexual contact, it is also the biggest sexually transmitted infection in both the United States and Europe. The prevalence in Sweden today is unknown as no national basic data exist. The routine procedure of identification is Wet-Smear although nucleic acid amplification enable a higher level of specificity and sensitivity. Panther™ System and GeneXpert® are two systems using RNA respectively DNA for analysis. The objective of the study was primarily to investigate the prevalence of Trichomonas vaginalis in STI-samples from Västra Götaland county with Aptima™ TV Assay on Panther™ System and secondarily to evaluate Aptima™ TV Assay with comparison to Xpert® TV Kit on GeneXpert®. The evaluation and prevalence study took place at Clinical Microbiology, Unilabs AB, Skaraborgs Hospital Skövde during the period of April-May 2017. The results of the evaluation indicate that Aptima™ TV Assay is specific for Trichomonas vaginalis and that the sensitivity falls within the detection limits for the kit. Therefore, the kit is suitable for use in the prevalence study. The prevalence study displayed a positive outcome on two out of 606 analyzed patient samples, corresponding to 0.3%. The conclusion is that the prevalence is enough for further studies.

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