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  • 1.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Studies on expression and regulation of phospholipase A2 and cyclooxygenase 2 in gastrointestinal tissues with special reference to colorectal cancer1999Doctoral thesis, monograph (Other scientific)
  • 2.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Dienus, Olaf
    Löfgren, Sture
    Hugander, Anders
    Wågsäter, Dick
    Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients2007In: International Journal of Oncology, ISSN 1019-6439, Vol. 31, no 1, p. 97-102Article in journal (Other academic)
  • 3.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Dienus, Olaf
    Löfgren, Sture
    Hugander, Anders
    Wågsäter, Dick
    Polymorphisms of Fractalkine receptor CX3CR1 and plasma levels of its ligand CX3CL1 in colorectal cancer patients2007In: International Journal of Colorectal Disease, ISSN 0179-1958, E-ISSN 1432-1262, Vol. 22, no 10, p. 1195-200Article in journal (Other academic)
  • 4.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Gustafson-Svärd, C
    Weström, B
    Tagesson, C
    Söderkvist, P
    Group I phospholipase A2 mRNA expression in rat glandular stomach and pancreas: Ontogenic development and effects of cortisone acetate.1992In: Biochimica et Biophysica Acta, ISSN 0006-3002, E-ISSN 1878-2434, Vol. 1130, no 1, p. 47-51Article in journal (Other academic)
  • 5.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, A
    Sirsjo, A
    Decreased levels of precursor transforming growth factor beta1 in human colorectal cancer.2001In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 7, no 6, p. 597-601Article in journal (Other academic)
  • 6.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, A
    Sirsjö, A
    Söderkvist, P
    Enhanced expression of cyclooxygenase-2 and nuclear beta-catenin are related to mutations in the APC gene in human colorectal cancer.2001In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 21, no 2A, p. 911-915Article in journal (Other academic)
  • 7.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, Anders
    Häll-Karlsson, Britt-Marie
    Sirsjö, Allan
    RFX-B, a MHC class II transcription factor, suppressed in human colorectal adenocarcinomas.2002In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 9, no 3, p. 213-216Article in journal (Other academic)
  • 8.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, Anders
    Löfgren, Sture
    Wågsäter, Dick
    Polymorphism and circulating levels of the chemokine CXCL12 in colorectal cancer patients2007In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 19, no 1, p. 11-15Article in journal (Refereed)
  • 9.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, Anders
    Wågsäter, Dick
    Expression of CD137 and CD137 ligand in colorectal cancer patients2006In: Oncology Reports, ISSN 1021-335X, E-ISSN 1791-2431, Vol. 15, no 5, p. 1197-1200Article in journal (Refereed)
  • 10.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, Anders
    Wågsäter, Dick
    Protein expression of the chemokine, CCL28, in human colorectal cancer.2006In: International Journal of Oncology, ISSN 1019-6439, Vol. 28, no 2, p. 315-319Article in journal (Other academic)
  • 11.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Lilja, I
    Weström, B
    Tagesson, C
    Söderkvist, P
    Gustafson-Svärd, C
    Ontogeny of group II phospholipase A2 gene expression in rat stomach and ileum.1995In: Biology of the Neonate, ISSN 0006-3126, E-ISSN 1421-9727, Vol. 67, no 2, p. 113-121Article in journal (Other academic)
  • 12.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Samuelsson, A
    Hugander, A
    Söderkvist, P
    Differential expression of cyclooxygenase 2 in human colorectal cancer.1999In: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 45, no 5, p. 730-732Article in journal (Other academic)
  • 13.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Samuelsson, A
    Hugander, A
    Söderkvist, P
    Gene expression of cyclooxygenase-2, group II and cytosolic phospholipase A2 in human colorectal cancer.1998In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 18, no 5A, p. 3283-3287Article in journal (Other academic)
  • 14.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Shamoun, Levar
    Department of Laboratory Medicine and Pathology, Region Jönköping County Hospital Ryhov, Jönköping, Sweden.
    Landerholm, Kalle
    Department of Surgery, Jönköping, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
    Andersson, Roland E.
    Department of Surgery, Jönköping, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
    Kolodziej, Blanka
    Department of Pathology, Region Jönköping County Hospital Ryhov, Jönköping, Sweden.
    Wågsäter, Dick
    Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
    Genetic variants of the IL2 gene related to risk and survival in patients with colorectal cancer2019In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 39, no 9, p. 4933-4940Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: Interleukin 2 (IL2) is a significant factor activating T-cell-mediated immune response by stimulation of natural killer cells, T-cells and in development of regulatory T (Treg) cells. Recent studies have that IL2 participates in cancer development by modifying the local immune response. Based on the suggested role of the single nucleotide polymorphisms (SNPs) rs2069762, rs6822844 and rs11938795 of IL2 in the pathogenesis of certain diseases, the relationship of these SNPs with clinicopathological variables and their possible implication for prognosis and disease outcome were evaluated in a cohort of Swedish patients with colorectal cancer (CRC).

    MATERIALS AND METHODS: TaqMan SNP genotype assays based on polymerase chain reaction were used for analysis of the IL2 SNPs in 467 patients with CRC and 467 healthy controls. Expression analysis of IL2 in plasma and CRC tissue was also performed.

    RESULTS: The allelic variants T in rs11938795 and G in rs6822844 were significantly associated with a higher risk of CRC. Kaplan-Meier analysis showed that cancer-specific survival was worse for individuals with C allele for rs2069762 with stage II CRC and with T allele for rs6822844 with stage III CRC.

    CONCLUSION: SNPs rs2069762, rs6822844 and rs11938795 of the IL2 gene may be helpful as prognostic biomarkers in the follow-up and management of the patients.

  • 15.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Skarstedt, Marita
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Löfgren, Sture
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Zar, Niklas
    Department of Surgery, Ryhov County Hospital, Jönköping, Sweden.
    Matussek, Andreas
    Department of Laboratory Services, Ryhov County Hospital, Jönköping, Sweden.
    Protein expression and gene polymorphism of CXCL10 in patients with colorectal cancer2014In: Biomedical Reports, ISSN 2049-9442, Vol. 2, no 3, p. 340-343Article in journal (Refereed)
    Abstract [en]

    Chemokines (chemotactic cytokines) promote leukocyte attraction to sites of inflammation and cancer. Certain chemokines promote and regulate neoplastic progression, including metastasis and angiogenesis. One such chemokine, CXCL10, was found to be expressed in colorectal cancer (CRC) tissue. To gain insight into the prognostic significance of CXCL10, we investigated whether the levels of this chemokine were altered in the colorectal tissue or plasma of CRC patients. Using Luminex technology for protein analyses, we observed a significantly higher CXCL10 protein level in cancer tissue compared to that in paired normal tissue. Moreover, significantly higher plasma levels of CXCL10 were detected in patients compared to those in control subjects and the plasma levels of CXCL10 in disseminated disease were found to be significantly higher compared to those in localized disease. The single‑nucleotide polymorphism rs8878, which has been described in exon 4 in the 3'‑untranslated region of the CXCL10 gene, was investigated using a TaqMan system. There were significant differences in genotype distribution and allelic frequencies between CRC patients and control subjects. In conclusion, altered CXCL10 protein concentrations in CRC tissues or plasma and the rs8878 genotype variant of CXCL10 may contribute to the prediction of clinical outcome.

  • 16.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Skarstedt, Marita
    Division of Medical Diagnostics, Department of Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
    Slind Olsen, Renate
    Division of Medical Diagnostics, Department of Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
    Andersson, Roland E.
    Department of Surgery, Region Jönköping County, Jönköping, Sweden.
    Matussek, Andreas
    Division of Medical Diagnostics, Department of Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
    Gene polymorphism in DNA repair genes XRCC1 and XRCC6 and association with colorectal cancer in Swedish patients2016In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 9, p. 736-740Article in journal (Refereed)
    Abstract [en]

    The DNA repair genes XRCC1 and XRCC6 have been proposed to participate in the pathological process of cancer by modulating the DNA repair capacity. This study evaluated the susceptibility of the single-nucleotide polymorphisms (SNPs) XRCC1 (rs25487, G > A) and XRCC6 (rs2267437, C > G) to colorectal cancer (CRC) and their association with clinical parameters in Swedish patients with CRC. Using the TaqMan system, these SNPs were screened in 452 patients and 464 controls. No significant difference in genotype distribution was found between the patients and controls, or any significant association with cancer-specific or disease-free survival in patients. However, we showed that the carriers of allele A in XRCC1 (rs25487, G > A) were connected with a higher risk of disseminated CRC (Odds Ratio = 1.64; 95% Confidence Interval = 1.12–2.41, p = 0.012).

  • 17.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Slind Olsen, Renate
    Department of Laboratory Services, Ryhov County Hospital, Jönköping.
    Skarstedt, Marita
    Clinical Microbiology, Ryhov County Hospital, Jönköping.
    Löfgren, Sture
    Department of Laboratory Services, Ryhov County Hospital, Jönköping.
    Zar, Niklas
    Ryhov County Hospital, Jönköping.
    Matussek, Andreas
    Department of Laboratory Services, Ryhov County Hospital, Jönköping.
    Polymorphism of the p38 beta gene in patients with colorectal cancer2014In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 8, p. 1093-1095Article in journal (Refereed)
    Abstract [en]

    The p38 mitogen‑activated protein kinase (MAPK) signaling pathways have been proposed to participate in the pathological process of cancer by affecting inflammation, proliferation, metastasis and cell survival. A single nucleotide polymorphism (SNP; rs2235356, ‑1628A→G) in the promoter region of the p38β gene has been proposed as a genetic modifier for colorectal cancer (CRC) in a Chinese population. The present study evaluated the susceptibility of patients possessing this SNP to CRC, in addition to determining its association with clinical parameters in Swedish patients with CRC. Using the LightSNiP genotyping assay, this SNP was screened in 389 patients with CRC and 517 control subjects. No significant difference in the genotype distribution or in the allelic frequencies was identified between the two groups nor was any association identified with the clinical parameters. These findings indicate that the ‑1628A→G polymorphism of the p38β gene is not significantly associated with a susceptibility to CRC in a Swedish population.

  • 18.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
    Ström, Karin
    Klinisk Mikrobiologi, Länssjukhuset Ryhov, Jönköping.
    Löfgren, Sture
    Klinisk Mikrobiologi, Länssjukhuset Ryhov, Jönköping.
    Zar, Niklas
    Kirurgkliniken, Länssjukhuset Ryhov, Jönköping.
    Hugander, Anders
    Kirurgkliniken, Länssjukhuset Ryhov, Jönköping.
    Matussek, Andreas
    Laboratoriemedicin, Länssjukhuset Ryhov, Jönköping.
    Expression of the serine protease inhibitor serpinA3 in human colorectal adenocarcinomas2011In: Oncology Letters, ISSN 1792-1074, Vol. 2, no 3, p. 413-418Article in journal (Refereed)
    Abstract [en]

    Proteases facilitate a number of steps in cancer progression. The serine protease inhibitors (serpins) are a protein superfamily with inhibitory activity against proteases. One of these proteases, serpinA3, appears to have a multifaceted role and is associated with inflammatory reactions, Alzheimer's disease, malignant melanoma and gastric cancer. To gain insight into the potential effect of serpinA3 on colorectal cancer (CRC) we determined whether serpinA3 is altered in colorectal tissue or plasma in CRC patients. Collectively, by using ELISA we noted a significantly lower serpinA3 level in cancer tissue compared to paired normal tissue. Moreover, the tumour serpinA3 level tended to be higher in disseminated disease as compared to localised disease. No significant difference in the plasma levels of serpinA3 was noted in the patients when compared to the controls. However, plasma serpinA3 and C-reactive protein (marker of inflammation) in the CRC patients and controls were significantly positively correlated. To confirm and detect localization of serpinA3 expression, immunohistochemistry was performed. Immunohistochemistry showed heterogeneous immunoreactivity in epithelial cells in the cancer and normal tissue and extracellular staining within bands of stroma as well as in some stromal cells. A Taq Man system was used to investigate a single nucleotide polymorphism (rs4934) in the serpinA3 signal sequence gene with supposed effect on serpinA3 secretion and expression. No significant difference was observed between CRC and control subjects regarding genotype and allelic distributions, nor were associations noted between clinical characteristics and serpinA3 levels. In conclusion, an altered serpinA3 concentration in CRC tissue may be a potential biomarker in CRC progression. SerpinA3 concentrations in plasma appear to be correlated with systemic inflammation, but do not appear to be specific to CRC patients. Further studies are warranted to improve our understanding of the role of serpinA3 in CRC.

  • 19.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Ström, Karin
    Dep. of Clinical Microbiology, Ryhov County Hospital Jönköping Sweden.
    Löfgren, Sture
    Dep. of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Zar, Niklas
    Dep. of Surgery, Ryhov County Hospital, Jönköping, Sweden.
    Lindh, Mikael
    Department of Surgery, Ryhov County Hospital, Jönköping, Sweden.
    Matussek, Andreas
    Dep. of Laboratory Medicin, Ryhov County Hospital, Jönköping, Sweden.
    DNA promoter methylation status and protein expression of interleukin-8 in human colorectal adenocarcinomas2012In: International Journal of Colorectal Disease, ISSN 0179-1958, E-ISSN 1432-1262, Vol. 27, no 6, p. 709-714Article in journal (Refereed)
    Abstract [en]

    Background  Interleukin-8 (IL-8) also referred to as CXCL8, a member of the CXC chemokine family that attracts neutrophils and other leukocytes, has been associated with cancer. Angiogenesis is a prime regulator of tumour expansion and data support that IL-8 is a potent angiogenic factor. Epigenomic instability has been postulated to play a role for the development of multiple neoplasias including colorectal cancer (CRC). DNA methylation of cytosine residues in CpG dinucleotides leads to transcriptional silencing of associated genes.

    Method  In this study, we comparatively analysed the protein expression of IL-8 in plasma, tumour and paired normal tissue and methylation status of the IL-8 gene to evaluate its impact on CRC.

    Results  Collectively, by using Luminex technology, we noted a significantly higher IL-8 level in cancer tissue compared to paired normal tissue and that CRC patients exhibit significantly higher plasma levels than healthy controls. Analysed by methylation-specific polymerase chain reaction, we detected IL-8 hypomethylation in 64% of the cancerous tissue cases but no hypomethylation was found in paired normal tissue. We noted that the CRC patients with IL-8 hypomethylation revealed a significant higher level of IL-8 protein in cancerous tissue, which tended to be associated with distant metastasis. We also observed that patients with distant metastasis showed a significantly higher plasma level of IL-8 in relation to patients without distant metastasis.

    Conclusion  Our results suggest that the predominance of high plasma levels of IL-8 in patients with distant metastasis in combination with the hypomethylation of the IL-8 promoter region might be a useful marker of the disease advancement.

  • 20.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Thai, Trinh Hong
    Key Laboratory of Enzyme and Protein Technology, College of Science, Vietnam National University, Hanoi, Vietnam.
    Skarstedt, Marita
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Löfgren, Sture
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Zar, Niklas
    Department of Surgery, Ryhov County Hospital, Jönköping, Sweden.
    Matussek, Andreas
    Department of Laboratory Medicine, Ryhov County Hospital, Jönköping, Sweden.
    Detection of Cytomegalovirus DNA in Colorectal Tissue from Swedish and Vietnamese Patients with Colorectal Cancer2013In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 33, no 11, p. 4947-4950Article in journal (Refereed)
    Abstract [en]

    Background: Human cytomegalovirus (HCMV) has been implicated as a factor, which might be associated with colorectal cancer (CRC) progression. Data from studies with HCMV-infected tumour cell lines have highlighted an oncomodulatory potential of HCMV. In the present study, we aimed to evaluate the prevalence of HCMV DNA in CRC tissue compared to matched normal tissue, and its association with clinical factors.

    Patients and Methods: We used quantitative real-time polymerase chain reaction assay to detect HCMV DNA in 202 cancerous and paired normal tissue from Swedish (n=119) and Vietnamese (n=83) CRC patients.

    Results: Overall, the HCMV DNA rate was significantly higher in cancerous in relation to paired normal tissue. Furthermore, a significantly higher frequency (39.8%) of HCMV DNA was observed in cancer tissues from the Vietnamese patients compared to the Swedish patients (15.1%). The prevalence of HCMV DNA in CRC tissue of 50% of those with disseminated disease tended to be higher compared to those with localized disease, with a prevalence of 33.3% in Vietnamese patients.

    Conclusion: Our observations indicate that the prevalence of HCMV DNA differs significantly between cancer and matched normal tissues. Thus, these data support a possible role of CMV in CRC. Moreover, we noted differences between Swedish and Vietnamese patients, indicating a role of ethnicity.

  • 21.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Thai, Trinh Hong
    Vietnam National University.
    Skarstedt, Marita
    Ryhov County Hospital, Jönköping.
    Löfgren, Sture
    Ryhov County Hospital, Jönköping.
    Zar, Niklas
    Ryhov County Hospital, Jönköping.
    Matussek, Andreas
    Ryhov County Hospital, Jönköping.
    Novel and Differential Accumulation of Mitochondrial DNA Deletions in Swedish and Vietnamese Patients with Colorectal Cancer2014In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 34, no 1, p. 147-152Article in journal (Refereed)
    Abstract [en]

    Background: Mitochondrial DNA (mtDNA) has been proposed to be involved in carcinogenesis and aging. The mtDNA 4977 bp deletion is one of the most frequently observed mtDNA mutations in human tissues and may play a role in colorectal cancer (CRC). In the present study, we aimed to evaluate the frequency of mtDNA 4977 bp deletion in CRC tissues and its association with clinical factors. Patients and Methods: We determined the presence of the 4977 bp common deletion in cancer and normal paired tissue samples from 105 Swedish and 88 Vietnamese patients with CRC using polymerase chain reaction (PCR) assays. Results: The mtDNA 4977 bp deletion was shown to be significantly more frequent in normal tissues in comparison with paired cancer tissues in both Swedish and Vietnamese patients. The 4977 bp common deletion was significantly more frequent in cancer tissues of the Vietnamese patients compared to the Swedish patients, and in Vietnamese cancer tissues, the 4977 bp deletion was significantly over represented in those with localized disease compared to those with disseminated disease. Moreover, we detected nine novel mtDNA deletions and found a significantly higher rate of these in CRC tissues in Swedish in comparison to Vietnamese patients. Conclusion: The mtDNA 4977 bp deletion seems to have an impact on the clinical outcome of CRC in Vietnamese patients, that the Swedish patients accumulate more of the detected novel deletions in CRC tissue compared to Vietnamese patients probably indicates divergent mechanisms in colorectal carcinogenesis.

  • 22.
    Dimberg, Jan
    et al.
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
    Trinh Hong, Thai
    Vietnam National University, Hanoi.
    Skarstedt, Marita
    Clinical Microbiology, Ryhov County Hospital Sweden.
    Löfgren, Sture
    Clinical Microbiology Ryhov County Hospital Sweden.
    Zar, Niklas
    Dep. of Surgery, Ryhov County Hospital Sweden.
    Matussek, Andreas
    Laboratory Services, Ryhov County Hospital Jönköping.
    Analysis of APC and IGFBP7 promoter gene methylation in Swedish and Vietnamese colorectal cancer patients2013In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 5, no 1, p. 25-30Article in journal (Refereed)
    Abstract [en]

    The tumour suppressor gene adenomatous polyposis coli (APC) is a key component that drives colorectal carcinogenesis. The reported DNA methylation in the promoter of APC varies greatly among studies of colorectal cancer (CRC) in different populations. Insulin-like growth factor binding protein 7 (IGFBP7), also known as IGFBP‑related protein 1 (IGFBP-rP1), is expressed in various tissue types, including the lung, brain, prostate and gastrointestinal tract, and has been suggested to play a tumour suppressor role against colorectal carcinogenesis. Studies have indicated that IGFBP7 is inactivated by DNA methylation in human colon, lung and breast cancer. In the present study, we used the methylation‑specific polymerase chain reaction to study the methylation status of the APC and IGFBP7 gene promoters in cancerous and paired normal tissue to evaluate its impact on clinical factors and association with ethnicity, represented by Swedish and Vietnamese CRC patients. We also investigated the distribution of CpG islands and the CpG dinucleotide density of each CpG island in the regions which were the subject of our investigation. Overall, normal tissue from Swedish patients exhibited a significantly higher frequency of IGFBP7 gene methylation in comparison with that of Vietnamese patients. Moreover, a significantly higher number of cancer tissues from Vietnamese individuals showed higher levels of methylation versus the paired normal tissue compared with that of Swedish patients. When we studied the methylation in cancer compared with the matched normal tissue in individuals, we found that a significantly higher number of Vietnamese patients had a higher degree of IGFBP7 gene methylation in cancer versus matched normal tissue in comparison with Swedish patients. Taken together, our results suggest that the methylation of the APC and IGFBP7 gene promoter region in cancerous tissue, in combination with the predominance of methylation in normal tissue, may serve as a prognostic factor in CRC patients.

  • 23. Elander, N
    et al.
    Zhou, J
    Ungerbäck, J
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Söderkvist, P
    Association between adenomatosis polyposis coli functional status and microsomal prostaglandin E synthethase-1 expression in colorectal cancer2009In: Molecular Carcinogenesis, ISSN 0899-1987, E-ISSN 1098-2744Article in journal (Refereed)
  • 24. Fransén, Karin
    et al.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Osterström, Anna
    Olsson, Anneli
    Söderkvist, Peter
    Sirsjö, Allan
    Nitric oxide synthase 2 mRNA expression in relation to p53 and adenomatous polyposis coli mutations in primary colorectal adenocarcinomas.2002In: Surgery, ISSN 0039-6060, E-ISSN 1532-7361, Vol. 131, no 4, p. 384-392Article in journal (Other academic)
  • 25. Fransén, Karin
    et al.
    Klintenäs, Maria
    Osterström, Anna
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Monstein, Hans-Jürg
    Söderkvist, Peter
    Mutation analysis of the BRAF, ARAF and RAF-1 genes in human colorectal adenocarcinomas.2004In: Carcinogenesis, ISSN 0143-3334, E-ISSN 1460-2180, Vol. 25, no 4, p. 527-533Article in journal (Other academic)
  • 26.
    Gabrielson, Marike
    et al.
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm.
    Vorkapic, Emina
    Department of Medical and Health Sciences, Linköping University.
    Folkesson, Maggie
    Department of Medical and Health Sciences, Linköping University.
    Welander, Martin
    Department of Medical and Health Sciences, Linköping University.
    Matussek, Andreas
    Department of Laboratory Services, County Hospital Ryhov.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Länne, Toste
    Department of Medical and Health Sciences, Linköping University.
    Skogberg, Josefin
    Division of Vascular Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm.
    Wågsäter, Dick
    Department of Medical and Health Sciences, Linköping University.
    Altered PPARγ coactivator-1 alpha expression in abdominal aortic aneurysm: Possible effects on mitochondrial biogenesis2016In: Journal of Vascular Research, ISSN 1018-1172, E-ISSN 1423-0135, Vol. 53, no 1-2, p. 17-26Article in journal (Refereed)
    Abstract [en]

    Introduction: Abdominal aortic aneurysm (AAA) is a complex and deadly vascular disorder. The pathogenesis of AAA includes destruction and phenotypic alterations of the vascular smooth muscle cells (VSMCs) and aortic tissues. PPARγ coactivator-1 alpha (PGC1α) regulates VSMC migration and matrix formation and is a major inducer of mitochondrial biogenesis and function, including oxidative metabolism. Methods: Protein and gene expression of PGC1α and markers for mitochondria biogenesis and cell type-specificity were analysed in AAA aortas from humans and mice and compared against control aortas. Results: Gene expression of PPARGC1A was decreased in human AAA and angiotensin (Ang) II-induced AAA in mice when compared to control vessels. However, high expression of PGC1α was detected in regions of neovascularisation in the adventitia layer. In contrast, the intima/media layer of AAA vessel exhibited defective mitochondrial biogenesis as indicated by low expression of PPARGC1A, VDAC, ATP synthase and citrate synthase. Conclusion: Our results suggest that mitochondrial biogenesis is impaired in AAA in synthetic SMCs in the media, with the exception of newly formed supporting vessels in the adventitia where the mitochondrial markers seem to be intact. To our knowledge, this is the first study investigating PGC1α and mitochondria biogenesis in AAA.

  • 27. Lilja, I
    et al.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Sjödahl, R
    Tagesson, C
    Gustafson-Svärd, C
    Effects of endotoxin and dexamethasone on group I and II phospholipase A2 in rat ileum and stomach.1994In: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 35, no 1, p. 40-45Article in journal (Other academic)
  • 28. Monstein, H-J
    et al.
    Fransén, K
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Söderkvist, P
    K-ras and B-raf gene mutations are not associated with gastrin- and CCK2-receptor mRNA expression in human colorectal tumour tissues.2004In: European Journal of Clinical Investigation, ISSN 0014-2972, E-ISSN 1365-2362, Vol. 34, no 2, p. 100-6Article in journal (Refereed)
  • 29. Mumtaz, Melad
    et al.
    Löfgren, Sture
    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Polymorphism in MHC class II transactivator gene is not associated with susceptibility to colorectal cancer in Swedish patients.2008In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 28, no 3B, p. 1789-1791Article in journal (Refereed)
  • 30. Mumtaz, Melad
    et al.
    Wågsäter, Dick
    Löfgren, Sture
    Hugander, Anders
    Zar, Niklas
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Decreased expression of the chemokine CCL21 in human colorectal cancer adenocarcinomas2009In: Oncology Reports, ISSN 1021-335X, E-ISSN 1791-2431, Vol. 21, no 1, p. 153-158Article in journal (Refereed)
  • 31. Mälarstig, Anders
    et al.
    Wågsäter, Dick
    Löfgren, Sture
    Hugander, Anders
    Zar, Niklas
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Tumour-derived adhesion factor in colorectal cancer2009In: Molecular Medicine Reports, ISSN 1791-2997, Vol. 2, no 6, p. 971-976Article in journal (Refereed)
    Abstract [en]

    Tumour-derived adhesion factor (TAF) has been shown to be associated with breast, prostate and colorectal cancer (CRC), acting as tumour suppressor or tumour promoter by mechanisms not as yet understood. Here, we comparatively analyzed the expression profile of TAF in plasma, tumour and paired normal tissue from patients with CRC. In addition, we investigated the relationship between TAF and systemic inflammation, mirrored by the elevation of interleukin-6 (IL-6) and TAF levels in plasma. Levels of TAF and IL-6 were determined by ELISA. Immunohistochemistry was performed to investigate the site of TAF expression. We also used a TaqMan system to investigate a TAF single nucleotide polymorphism (rs2041437) with a potential effect on CRC. TAF protein levels were significantly (P<0.001) higher in colorectal tumours than in normal tissue, and were increased in patients with Dukes' stages B and C compared to A. Immunohistochemistry revealed heterogeneous TAF expression mainly in the epithelial cells of the cancer and normal tissue. The plasma TAF level was reduced in CRC patients compared with the controls (P=0.002), independent of the inflammatory marker IL-6. Regarding genotype and allelic distributions, significant differences between CRC patients and control subjects or associations between clinical characteristics and TAF levels in tissue and plasma were not observed. In conclusion, altered TAF protein expression in cancer tissue may be a potential biomarker in colorectal carcinogenesis. Further research exploring the regulation of TAF is required to evaluate whether TAF is linked to clinical outcome.

  • 32.
    Olsen, Renate S.
    et al.
    Division of Medical Diagnostics, Department of Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
    Nijm, Johnny
    Division of Medical Diagnostics, Department of Clinical Physiology, Region Jönköping County, Jönköping, Sweden.
    Andersson, Roland E.
    Department of Surgery, Region Jönköping County, Jönköping, Sweden.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Wågsäter, Dick
    Division of Drug Research, Department of Medicine and Health Sciences, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden.
    Circulating inflammatory factors associated with worse long-term prognosis in colorectal cancer2017In: World Journal of Gastroenterology, ISSN 1007-9327, E-ISSN 2219-2840, Vol. 23, no 34, p. 6212-6219Article in journal (Refereed)
    Abstract [en]

    AIM: To investigate association of circulating inflammatory factors at the time of colorectal cancer (CRC) surgery with survival.

    METHODS: Plasma levels from 174 CRC patients (69 females and 105 men), with median age 70 years (range 29-90), localized in the colon (n = 105) or rectum (n = 69), with stage I (n = 24), stage II (n = 54), stage III (n = 67) and stage IV (n = 29) were measured using commercially available Bio-Plex Pro™ Human Chemokine Panel 40-Plex, including 40 different chemokines, cytokines and interleukins. The prognostic association of each inflammatory factor was analysed as CRC-specific and total mortality.

    RESULTS: Out of 174 patients, 66 died during the follow-up, 40 because of CRC specific mortality. High tertile levels of 8 factors were significantly associated with increased CRC-specific mortality, of which CCL1, CCL20, CCL24, CX3CL1, IL-4 and TNF-α remained significant in a multivariate Cox regression analysis. High tertile levels of 14 factors were associated with increased total mortality, of which CCL1, CCL15, CCL20, CX3CL1, CXCL13, IFN-γ, IL-2, IL-4 and IL-10 remained significant after adjustment for clinical covariates. For most of the inflammatory factors the association between higher tertile levels and an increased mortality in general appeared two years after surgery. High tertile levels of TNF-α and CCL24 were exclusively associated with CRC-specific mortality. The distribution of these factors were not associated with TNM stage with exception for CCL20.

    CONCLUSION: High plasma levels of inflammatory factors are associated with increased risk of mortality among CRC patients and could be potential biomarkers for revealing prognosis.

  • 33.
    Olsen, Renate Slind
    et al.
    Department of Laboratory Medicine, Division of Medical Diagnostics, Region Jönköping County, Jönköping, Sweden.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Geffers, Robert
    Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    Wågsäter, Dick
    Division of Drug Research, Department of Medicine and Health Sciences, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden.
    Possible role and therapeutic target of PDGF-D signalling in colorectal cancer2019In: Cancer Investigation, ISSN 0735-7907, E-ISSN 1532-4192, Vol. 37, no 2, p. 99-112Article in journal (Refereed)
    Abstract [en]

    Platelet-derived growth factor D (PDGF-D) has been shown to mediate cellular processes of importance in cancer progression. This study aimed to investigate the expression and putative involvement of PDGF-D signaling in colorectal carcinogenesis. PDGF-D was expressed in vascular endothelial cells in tumor and normal tissues. PDGF-D stimulation of cells altered genes of importance in carcinogenic processes. In addition, PDGF-D increased the proliferation rate while imatinib inhibited these effects. PDGF-D and its PDGF receptor beta (PDGFR-β) are expressed in colorectal cancer and blockage of PDGF-D/PDGFR-β signaling using tyrosine kinase inhibitors, such as imatinib, might be important in inhibiting tumor-promoting actions.

  • 34. Osterström, Anna
    et al.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Fransén, Karin
    Söderkvist, Peter
    Expression of cytosolic and group X secretory phospholipase A(2) genes in human colorectal adenocarcinomas.2002In: Cancer Letters, ISSN 0304-3835, E-ISSN 1872-7980, Vol. 182, no 2, p. 175-182Article in journal (Refereed)
  • 35.
    Shamoun, Levar
    et al.
    Division of Medical Diagnostics, Department of Laboratory Medicine, Jönköping County, Jönköping, Sweden.
    Kolodziej, Blanka
    Department of Pathology, Jönköping County, Jönköping, Sweden.
    Andersson, Roland E.
    Department of Surgery, Jönköping County, Jönköping, Sweden.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Protein expression and genetic variation of IL32 and association with colorectal cancer in Swedish patients2018In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 38, no 1, p. 321-328Article in journal (Refereed)
    Abstract [en]

    Background: Interleukin 32 (IL32) is an intracellular pluripotent cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells and seems to be involved in the pathogenesis of cancer and inflammatory diseases. Our purpose was to assess the role of protein expression and genetic polymorphisms of IL32 in colorectal cancer (CRC) susceptibility.

    Materials and Methods: To gain insight into clinical significance of IL32 in Swedish patients with CRC, using enzyme-linked immunosorbent assay, we determined whether IL32 protein level is altered in CRC tissue (n=75) compared with paired normal tissue and in plasma from patients with CRC (n=94) compared with controls (n=81). The expression of IL32 protein was confirmed by immunohistochemistry (n=73). We used Luminex technology to investigate protein levels of the cytokines IL6, tumor necrosis factor-a (TNFa) and vascular endothelial growth factor (VEGF) to relate these to IL32 levels in CRC tissue. Three single nucleotide polymorphisms (SNPs) (rs28372698, rs12934561, rs4786370) of the IL32 gene have been proposed as modifiers for different diseases. The present study evaluated the susceptibility of patients possessing these SNPs to CRC. Using TaqMan SNP genotyping assays, these SNPs were screened in Swedish patients with CRC (n=465) and healthy controls (n=331).

    Results: We found no significant differences in the genotypic frequencies between the patients and healthy controls and no relation to survival for any of the SNPs. However, the SNP rs12934561 was statisticalLY significant associated with older patients. IL32 protein was up-regulated in CRC tissue and related to IL6, TNFa, and VEGF, and seems to be modulated by SNP rs28372698. The IL32 protein level in CRC tissue also reflects both disseminated disease and location. Conclusion. Our results suggest that altered IL32 protein concentrations in CRC tissue and genotypic variants of IL32 are related to disseminated CRC.

  • 36.
    Shamoun, Levar
    et al.
    Department of Laboratory Medicine, Region Jönköping County, Jönköping, Sweden.
    Skarstedt, Marita
    Department of Laboratory Medicine, Division of Clinical Microbiology, Region Jönköping County, Jönköping, Sweden.
    Andersson, Roland E.
    Depatment of Surgery, Region Jönköping County, Jönköping, Sweden.
    Wågsäter, Dick
    Division of Drug Research, Department of Medicine and Health Sciences Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Association study on IL-4, IL-4Rα and IL-13 genetic polymorphisms in Swedish patients with colorectal cancer2018In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 487, p. 101-106Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: Interleukin 4 (IL-4) and interleukin 13 (IL-13) are anti-inflammatory and immunomodulatory cytokines which share a common cellular receptor IL4Rα and are involved in the same signaling pathways. Our purpose was to assess whether genetic variants within IL-4, IL-13 and IL-4Rα are associated with the risk or clinical outcome of colorectal cancer (CRC).

    METHODS: Three single nucleotide polymorphisms (SNPs) were screened in 466 patients with CRC and 445 healthy controls. The selected SNPs were IL-4 SNP rs2243250, IL-4Rα SNP rs1801275 and IL-13 SNP rs1800925.

    RESULTS: We found that the genotype variant T/T in IL-13 gene was associated with a higher risk of CRC. Kaplan-Meier analysis showed that the cancer specific survival differed between C/C and CT + TT for IL-4 SNP. Moreover, the carriers of the T allele were associated with the highest risk of CRC death with a hazard ratio (HR) of 1.57, 95% CI 1.06-2.36, p = .024. The observed effect of the T allele was restricted to stage III patients.

    CONCLUSION: Our results indicate IL-13 SNP rs1800925 as a risk factor for CRC and that IL-4 SNP rs2243250 could be a useful prognostic marker in the follow-up and clinical management of patients with CRC especially in stage III disease.

  • 37.
    Song, Nguyen Van
    et al.
    Department of Medical Laboratory, Da Nang University of Medical Technology and Pharmacy, Da Nang, Vietnam.
    Minh, Nguyen Khac
    Department of Medical Laboratory, Da Nang University of Medical Technology and Pharmacy, Da Nang, Vietnam.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
    Matussek, Andreas
    Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden.
    Henningsson, Anna J.
    Clinical Microbiology, Division of Medical Diagnostics, Region Jönköping County, Jönköping, Sweden.
    Prevalence of cervical infection and genotype distribution of human Papilloma virus among females in Da Nang, Vietnam2017In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 37, no 3, p. 1243-1247Article in journal (Refereed)
    Abstract [en]

    Aim: The goal of the present study was to determine the prevalence and distribution of high-risk human papilloma virus (HPV) genotypes in women from two districts in and around Da Nang city, Vietnam.

    Materials and Methods: All participants were randomly selected, 200 from the Hai Chau district and 200 from the Son Tra district. The detection and genotyping of HPV were performed by real-time polymerase chain reaction (PCR) technique.

    Results: Out of a total of 400 women, we found that 38 (9.5%) were infected with a high-risk HPV genotype, the most prevalent genotypes being 16, 18, 58 and 59. By assessment of the HPV findings in relation to sociodemographic characteristics, we found significant differences between the two study districts and between the age groups, as well as differences associated with occupation and the use of contraceptives.

    Conclusion: The proportion of high–risk genotypes other than 16 and 18 was relatively high, and since the HPV genotype distribution is known to vary greatly across populations, the information from this study can be used for planning of screening and vaccination programs in Da Nang.

  • 38.
    Song Van, Nguyen
    et al.
    Da Nang Univ Med Technol & Pharm, Dept Lab Med, Da Nang, Vietnam.
    Skarstedt, Marita
    Ryhov Cty Hosp, Dept Clin Microbiol, Jonkoping, Sweden.
    Löfgren, Sture
    Ryhov Cty Hosp, Dept Clin Microbiol, Jonkoping, Sweden.
    Zar, Niklas
    Ryhov Cty Hosp, Dept Surg, Jonkoping, Sweden.
    Andersson, Roland E
    Ryhov Cty Hosp, Dept Surg, Jonkoping, Sweden.
    Lindh, Mikael
    Ryhov Cty Hosp, Dept Surg, Jonkoping, Sweden.
    Matussek, Andreas
    Ryhov Cty Hosp, Dept Lab Med, Jonkoping, Sweden.
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Gene Polymorphism of Matrix Metalloproteinase-12 and-13 and Association with Colorectal Cancer in Swedish Patients2013In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 33, p. 3247-3250Article in journal (Refereed)
    Abstract [en]

    Background: It has been widely reported that matrix metalloproteinases (MMPs) have fundamental roles in pathological processes in cancer through degradation of basal membranes and extracellular matrix. For MMP12 and MMP13, a functional single nucleotide polymorphism (SNP) has been detected -82A -> G (rs2276109) and -77A -> G (rs2252070), respectively. These SNPs are suggested to have an influence on different diseases. The present study evaluated the association between these SNPs in patients with colorectal cancer (CRC) patients and healthy controls. Patients and Methods: Using the TaqMan system, these SNPs were screened in 385 patients with CRC and 619 controls. Results: No significant difference in genotype distribution or in allelic frequencies was found between the two groups. However, we showed that the AA MMP-12 genotype is connected with a higher risk of disseminated CRC (Odds Ratio=1.77; 95% Confidence Interval=1.11-2.81, p=0.018). Conclusion: The results of this study suggest that the -82A -> G (rs2276109) polymorphism of the MMP12 gene reflects clinical outcome of patients with CRC.

  • 39. Ungerbäck, Jonas
    et al.
    Elander, Nils
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Söderkvist, Peter
    Analysis of VEGF polymorphisms, tumor expression of VEGF mRNA and colorectal cancer susceptibility in a Swedish population2009In: Molecular Medicine Reports, ISSN 1791-2997, Vol. 2, no 3, p. 435-439Article in journal (Refereed)
    Abstract [en]

    Vascular endothelial growth factor (VEGF) plays a significant role in tumor angiogenesis and is found to be overexpressed and involved in the development and progression of colorectal cancer (CRC). The VEGF gene contains several polymorphic sites known to influence VEGF expression. We examined the possible association between five polymorphisms, located in the promoter/5'-untranslated region [-2578 (C/A), -2549 (del/ins 18 bp) -1154 (G/A), -634 (G/C)] or 3'-untranslated region [+936 (C/T)] of the VEGF gene, and CRC Susceptibility and clinicopathological characteristics in 302 Swedish CRC patients and 336 healthy randomly selected controls. Both genotypes and combined haplotypes were analyzed. No significant differences were observed when VEGF genotype/haplotype frequencies in the CRC cases and controls were compared, nor were any associations found between the genotypes/haplotypes and clinicopathological characteristics. However, when the -2578 C and +936 T alleles were combined, a small but significant association with CRC susceptibility was detected (OR=1.6, 95% CI 1.3-1.9, p=0.01). In addition, VEGF mRNA expression was determined in a Subset of patients, revealing a 2-fold VEGF upregulation in CRC tissue compared to normal colonic mucosa, but no association between the genotypes or haplotypes and VEGF mRNA levels. Linkage analysis was performed, revealing that the polymorphisms in the promoter and 5'-untranslated region were in tight linkage disequilibrium (LD) (vertical bar D'vertical bar=0.91-1.00), while the +936 C/T polymorphism was only weakly associated with the others (vertical bar D'vertical bar=0.05-0.19). In conclusion, VEGF is generally upregulated in colorectal tumors. However, the single nucleotide polymorphisms examined do not appear to influence the mRNA expression of VEGF in colorectal tumors, and most likely play a limited role in CRC development and progression.

  • 40. Viet, Hung Trinh
    et al.
    Wågsäter, Dick
    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Interleukin-1 receptor antagonist gene polymorphism in human colorectal cancer.2005In: Oncology Reports, ISSN 1021-335X, E-ISSN 1791-2431, Vol. 14, no 4, p. 915-918Article in journal (Other academic)
  • 41. Wägsäter, Dick
    et al.
    Löfgren, Sture
    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Expression of interleukin-17 in human colorectal cancer2006In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 26, no 6B, p. 4213-4216Article in journal (Refereed)
  • 42. Wågsater, Dick
    et al.
    Mumtaz, Melad
    Lofgren, Sture
    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Ageing - living conditions and health. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Resistin in Human Colorectal Cancer: Increased Expression Independently of Resistin Promoter -420C>G genotype.2008In: Cancer Investigation, ISSN 0735-7907, E-ISSN 1532-4192, Vol. 26, no 10, p. 1008-1014Article in journal (Refereed)
    Abstract [en]

    A single nucleotide polymorphism (SNP) -420C> G of the resistin gene was screened in 248 colorectal cancer (CRC) patients and 256 controls. No significant difference in genotype distribution was found. However, we found an upregulation in 92% of the samples in the levels of resistin protein in cancer tissue (n = 83). Immunohistochemical analysis revealed heterogenous staining of resistin predominantly in the cancer tissue. Further, resistin induced secretion of MMP-2 and MMP-9 from monocytes. The results of this study suggest that resistin may play a partial role in CRC but that the -420C> G resistin polymorphism is not a potential genetic susceptibility factor.

  • 43. Wågsäter, D
    et al.
    Sirsjö, A
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Down-regulation of ID2 by all-trans retinoic acid in monocytic leukemia cells (THP-1)2003In: Journal of Experimental and Clinical Cancer Research, ISSN 1756-9966, E-ISSN 1756-9966, Vol. 22, no 3, p. 471-475Article in journal (Other academic)
  • 44. Wågsäter, Dick
    et al.
    Dienus, Olaf
    Löfgren, Sture
    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health and Welfare, HHJ. Ageing - living conditions and health. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
    Quantification of the chemokines CCL17 and CCL22 in human colorectal adenocarcinomas2008In: Molecular Medicine Reports, ISSN 1791-2997, Vol. 1, no 2, p. 211-217Article in journal (Refereed)
    Abstract [en]

    Chemokines are believed to play a crucial role in local immunoresponse by regulating leukocyte movement in various tissues, including the intestinal mucosa. It has been suggested that they are key players in cancer biology, and several studies have identified leukocyte infiltration as a hallmark of most cancers. The chemokines CCL17 and CCL22 attract CCR4-bearing cells, which are especially polarised to Th2-type cells and regulatory T cells (Treg). Recent studies have revealed the participation of the CCL17 and CCL22 proteins in diseases such as atopic dermatitis and lymphoma. The purpose of this study was to assess the role of CCL17 and CCL22 protein expression in colorectal cancer (CRC) and to ascertain whether an association exists between promoter -431C>T CCL17 and -961G>A CCL22 gene polymorphisms in CRC versus non-CRC subjects. Using the ELISA assay, we noted a significantly higher expression of CCL22 in tumour tissue with a 2.3-fold up-regulation (tumour vs. paired normal tissue, n=78) but no significant difference in CCL17 protein expression. Immunohistochemistry revealed protein expression of CCL22 and CCL17 in the epithelial compartment of cancer tissue, in epithelial cells at the resection border that reflects normal tissue, and in some stromal cells such as lymphocytes, macrophages, and fibroblasts. Using a TaqMan system we screened for -431C>T CCL17 and -961G>A CCL22 gene variants in 245 CRC patients and 256 controls, but could not find any significant difference in genotype distribution or in allelic frequencies between the two groups. The genotype and allelic distributions of CRC patients were not related to tissue levels of CCL17 and CCL22 protein, and none of the variables were associated with plasma levels or clinical characteristics. To ascertain whether the tissue expression of CCL17 and CCL22 exerts an influence oil the pathogenesis of CRC, a forthcoming study oil the 5-year survival rate of CRC patients will be conducted.

  • 45. Wågsäter, Dick
    et al.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Expression of chemokine receptor CXCR6 in human colorectal adenocarcinomas.2004In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 24, no 6, p. 3711-3714Article in journal (Refereed)
  • 46. Wågsäter, Dick
    et al.
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Hugander, Anders
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health.
    Sirsjö, Allan
    Ghaderi, Mehran
    Analysis of MICA gene transcripts in human rectal cancers2003In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 23, no 3B, p. 2525-2529Article in journal (Other academic)
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    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Sirsjö, Allan
    Induction of ATP-binding cassette A1 by all-trans retinoic acid: possible role of liver X receptor-alpha.2003In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 11, no 4, p. 419-423Article in journal (Other academic)
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    Hugander, Anders
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Expression of CXCL16 in human rectal cancer.2004In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 14, no 1, p. 65-69Article in journal (Other academic)
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    Jatta, Ken
    Ocaya, Pauline
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Sirsjo, Allan
    Expression of IL-1beta, IL-1 receptor type I and IL-1 receptor antagonist in human aortic smooth muscle cells: effects of all-trans-retinoic acid.2006In: Journal of Vascular Research, ISSN 1018-1172, E-ISSN 1423-0135, Vol. 43, no 4, p. 377-382Article in journal (Other academic)
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    Hugander, Anders
    Dienus, Olaf
    Dimberg, Jan
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Ageing - living conditions and health. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
    Analysis of single nucleotide polymorphism in the promoter and protein expression of the chemokine eotaxin-1 in colorectal cancer patients2007In: World Journal of Surgical Oncology, ISSN 1477-7819, E-ISSN 1477-7819, Vol. 5, p. 84-Article in journal (Refereed)
12 1 - 50 of 52
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