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  • 1. Antoniou, A. C.
    et al.
    Sinilnikova, O. M.
    McGuffog, L.
    Healey, S.
    Nevanlinna, H.
    Heikkinen, T.
    Simard, J.
    Spurdle, A. B.
    Beesley, J.
    Chen, X.
    Neuhausen, S. L.
    Ding, Y. C.
    Couch, F. J.
    Wang, X.
    Fredericksen, Z.
    Peterlongo, P.
    Peissel, B.
    Bonanni, B.
    Viel, A.
    Bernard, L.
    Radice, P.
    Szabo, C. I.
    Foretova, L.
    Zikan, M.
    Claes, K.
    Greene, M. H.
    Mai, P. L.
    Rennert, G.
    Lejbkowicz, F.
    Andrulis, I. L.
    Ozcelik, H.
    Glendon, G.
    Gerdes, A. -M
    Thomassen, M.
    Sunde, L.
    Caligo, M. A.
    Laitman, Y.
    Kontorovich, T.
    Cohen, S.
    Kaufman, B.
    Dagan, E.
    Baruch, R. G.
    Friedman, E.
    Harbst, K.
    Barbany-Bustinza, G.
    Rantala, J.
    Ehrencrona, H.
    Karlsson, P.
    Domchek, S. M.
    Nathanson, K. L.
    Osorio, A.
    Blanco, I.
    Lasa, A.
    Benítez, J.
    Hamann, U.
    Hogervorst, F. B. L.
    Rookus, M. A.
    Collee, J. M.
    Devilee, P.
    Ligtenberg, M. J.
    van der Luijt, R. B.
    Aalfs, C. M.
    Waisfisz, Q.
    Wijnen, J.
    van Roozendaal, C. E. P.
    Peock, S.
    Cook, M.
    Frost, D.
    Oliver, C.
    Platte, R.
    Evans, D. G.
    Lalloo, F.
    Eeles, R.
    Izatt, L.
    Davidson, R.
    Chu, C.
    Eccles, D.
    Cole, T.
    Hodgson, S.
    Godwin, A. K.
    Stoppa-Lyonnet, D.
    Buecher, B.
    Léoné, M.
    Bressac-de Paillerets, B.
    Remenieras, A.
    Caron, O.
    Lenoir, G. M.
    Sevenet, N.
    Longy, M.
    Ferrer, S. F.
    Prieur, F.
    Goldgar, D.
    Miron, A.
    John, E. M.
    Buys, S. S.
    Daly, M. B.
    Hopper, J. L.
    Terry, M. B.
    Yassin, Y.
    Singer, C.
    Gschwantler-Kaulich, D.
    Staudigl, C.
    Hansen, T. V. O.
    Barkardottir, R. B.
    Kirchhoff, T.
    Pal, P.
    Kosarin, K.
    Offit, K.
    Piedmonte, M.
    Rodriguez, G. C.
    Wakeley, K.
    Boggess, J. F.
    Basil, J.
    Schwartz, P. E.
    Blank, S. V.
    Toland, A. E.
    Montagna, M.
    Casella, C.
    Imyanitov, E. N.
    Allavena, A.
    Schmutzler, R. K.
    Versmold, B.
    Engel, C.
    Meindl, A.
    Ditsch, N.
    Arnold, N.
    Niederacher, D.
    Deißler, H.
    Fiebig, B.
    Suttner, C.
    Schönbuchner, I.
    Gadzicki, D.
    Caldes, T.
    de la Hoya, M.
    Pooley, K. A.
    Easton, D. F.
    Chenevix-Trench, G.
    Common variants in LSP1, 2q35 and 8q24 and breast cancer risk for BRCA1 and BRCA2 mutation carriers2009In: Human Molecular Genetics, ISSN 0964-6906, E-ISSN 1460-2083, Vol. 18, no 22, p. 4442-4456Article in journal (Refereed)
    Abstract [en]

    Genome-wide association studies of breast cancer have identified multiple single nucleotide polymorphisms (SNPs) that are associated with increased breast cancer risks in the general population. In a previous study, we demonstrated that the minor alleles at three of these SNPs, in FGFR2, TNRC9 and MAP3K1, also confer increased risks of breast cancer for BRCA1 or BRCA2 mutation carriers. Three additional SNPs rs3817198 at LSP1, rs13387042 at 2q35 and rs13281615 at 8q24 have since been reported to be associated with breast cancer in the general population, and in this study we evaluated their association with breast cancer risk in 9442 BRCA1 and 5665 BRCA2 mutation carriers from 33 study centres. The minor allele of rs3817198 was associated with increased breast cancer risk only for BRCA2 mutation carriers [hazard ratio (HR) = 1.16, 95% CI: 1.07-1.25, P-trend = 2.8 × 10-4]. The best fit for the association of SNP rs13387042 at 2q35 with breast cancer risk was a dominant model for both BRCA1 and BRCA2 mutation carriers (BRCA1: HR = 1.14, 95% CI: 1.04-1.25, P = 0.0047; BRCA2: HR = 1.18 95% CI: 1.04-1.33, P = 0.0079). SNP rs13281615 at 8q24 was not associated with breast cancer for either BRCA1 or BRCA2 mutation carriers, but the estimated association for BRCA2 mutation carriers (per-allele HR = 1.06, 95% CI: 0.98-1.14) was consistent with odds ratio estimates derived from population-based case-control studies. The LSP1 and 2q35 SNPs appear to interact multiplicatively on breast cancer risk for BRCA2 mutation carriers. There was no evidence that the associations vary by mutation type depending on whether the mutated protein is predicted to be stable or not. 

  • 2.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, Lund University, Lund, Sweden.
    Calmodulin-stimulated Ca2+-ATPases in the vacuolar and plasma membranes in cauliflower1997In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 114, no 3, p. 999-1007Article in journal (Refereed)
    Abstract [en]

    The subcellular locations of Ca2+-ATPases in the membranes of cauliflower (Brassica oleracea L.) inflorescences were investigated. After continuous sucrose gradient centrifugation a 111-kD calmodulin (CaM)-stimulated and CaM-binding Ca2+-ATPase (BCA1; P. Askerlund [1996] Plant Physiol 110: 913–922; S. Malmstrom, P. Askerlund, M.G. Palmgren [1997] FEBS Lett 400: 324–328) comigrated with vacuolar membrane markers, whereas a 116-kD CaM-binding Ca2+-ATPase co-migrated with a marker for the plasma membrane. The 116-kD Ca2+-ATPase was enriched in plasma membranes obtained by aqueous two-phase partitioning, which is in agreement with a plasma membrane location of this Ca2+-ATPase. Countercurrent distribution of a low-density intracellular membrane fraction in an aqueous two-phase system resulted in the separation of the endoplasmic reticulum and vacuolar membranes. The 111-kD Ca2+-ATPase co-migrated with a vacuolar membrane marker after countercurrent distribution but not with markers for the endoplasmic reticulum. A vacuolar membrane location of the 111-kD Ca2+-ATPase was further supported by experiments with isolated vacuoles from cauliflower: (a) Immunoblotting with an antibody against the 111-kD Ca2+-ATPase showed that it was associated with the vacuoles, and (b) ATP-dependent Ca2+ uptake by the intact vacuoles was found to be CaM stimulated and partly protonophore insensitive.

  • 3.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, Lund University, Lund, Sweden.
    Modulation of an Intracellular Calmodulin-Stimulated Ca2+-Pumping ATPase in Cauliflower by Trypsin (The Use of Calcium Green-5N to Measure Ca2+ Transport in Membrane Vesicles)1996In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 110, no 3, p. 913-922Article in journal (Refereed)
    Abstract [en]

    The effect of controlled trypsin digestion of a calmodulin-stimulated Ca2+-ATPase in low-density intracellular membranes from cauliflower (Brassica oleracea L.) inflorescences was investigated. Ca2+ uptake into vesicles was measured either continuously with the fluorescent Ca2+ indicator Calcium Green-5N or with a radio-active filter technique. Trypsin treatment of vesicles resulted in a 3-fold activation of Ca2+ uptake and loss of calmodulin sensitivity. Immunoblotting experiments with an antiserum raised against the Ca2+-ATPase showed that the trypsin activation was accompanied by a decrease in the amount of intact Ca2+-ATPase (111 kD) and by successive appearances of polypeptides of 102 and 99 to 84 kD. 125I-Calmodulin overlays showed that only the intact Ca2+-ATPase bound calmodulin. Removal of the calmodulin-binding domain (about 9 kD) was not enough to obtain full activation. Trypsin proteolysis resulted in a Ca2+ concentration necessary for half-maximal activity of 0.5 [mu]M, whereas a value of about 2 [mu]M was obtained with untreated membranes in the presence of calmodulin. Without trypsin treatment or calmodulin the activity was not saturated even at 57 [mu]M free Ca2+. The data suggest that trypsin digestion and calmodulin activate the cauliflower Ca2+-ATPase by at least partly different mechanisms.

  • 4.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research.
    Evans, David
    Detection of distinct phosphorylated intermediates of Ca2+-ATPase and H+-ATPase in plasma membranes from Brassica oleracea1993In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 31, no 5, p. 787-791Article in journal (Refereed)
    Abstract [en]

    Two distinct phosphorylated intermediates representing the Ca2+-ATPase and H+-ATPase, respectively, were detected after phosphorylation of plasma membranes from cauliflower (Brassica oleracea L.) inflorescences with [gammaP-32]ATP and separation of polypeptides in an acidic gel. A 116 kDa polypeptide was identified as a Ca2+-ATPase by its Ca2+-dependent phosphorylation which was enhanced by La3+. A second polypeptide (105 kDa) also phosphorylated in the absence of Ca2+ and was identified as the H+-ATPase by immune blotting.

  • 5.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Sciences, University of Oxford, Oxford, United Kingdom.
    Evans, David E.
    Department of Plant Sciences, University of Oxford, Oxford, United Kingdom.
    Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L1992In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 100, no 4, p. 1670-1681Article in journal (Refereed)
    Abstract [en]

    Purification and functional reconstitution of a calmodulin-stimulated Ca(2+)-ATPase from cauliflower (Brassica oleracea L.) is described. Activity was purified about 120-fold from a microsomal fraction using calmodulin-affinity chromatography. The purified fraction showed a polypeptide at 115 kD, which formed a phosphorylated intermediate in the presence of Ca(2+), together with a few polypeptides with lower molecular masses that were not phosphorylated. The ATPase was reconstituted into liposomes by 3-([cholamidopropyl]-dimethylammonio-)1-propanesulfonate (CHAPS) dialysis. The proteoliposomes showed ATP-dependent Ca(2+) uptake and ATPase activity, both of which were stimulated about 4-fold by calmodulin. Specific ATPase activity was about 5 mumol min(-1) (mg protein)(-1), and the Ca(2+)/ATP ratio was 0.1 to 0.5 when the ATPase was reconstituted with entrapped oxalate. The purified, reconstituted Ca(2+)-ATPase was inhibited by vanadate and erythrosin B, but not by cyclopiazonic acid and thapsigargin. Activity was supported by ATP (100%) and GTP (50%) and had a pH optimum of about 7.0. The effect of monovalent and divalent cations (including Ca(2+)) on activity is described. Assay of membranes purified by two-phase partitioning indicated that approximately 95% of the activity was associated with intracellular membranes, but only about 5% with plasma membranes. Sucrose gradient centrifugation suggests that the endoplasmic reticulum is the major cellular location of calmodulin-stimulated Ca(2+)-pumping ATPase in Brassica oleracea inflorescences.

  • 6.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, University of Lund, Lund, Sweden.
    Larsson, Christer
    Department of Plant Biochemistry, University of Lund, Lund, Sweden.
    Transmembrane Electron Transport in Plasma Membrane Vesicles Loaded with an NADH-Generating System or Ascorbate1991In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 96, no 4, p. 1178-1184Article in journal (Refereed)
    Abstract [en]

    Sugar beet (Beta vulgaris L.) leaf plasma membrane vesicles were loaded with an NADH-generating system (or with ascorbate) and were tested spectrophotometrically for their ability to reduce external, membrane-impermeable electron acceptors. Either alcohol dehydrogenase plus NAD+ or 100 millimolar ascorbate was included in the homogenization medium, and right-side-out (apoplastic side-out) plasma membrane vesicles were subsequently prepared using two-phase partitioning. Addition of ethanol to plasma membrane vesicles loaded with the NADH-generating system led to a production of NADH inside the vesicles which could be recorded at 340 nanometers. This system was able to reduce 2,6-dichlorophenolindophenol-3′-sulfonate (DCIP-sulfonate), a strongly hydrophilic electron acceptor. The reduction of DCIP-sulfonate was stimulated severalfold by the K+ ionophore valinomycin, included to abolish membrane potential (outside negative) generated by electrogenic transmembrane electron flow. Fe3+-chelates, such as ferricyanide and ferric citrate, as well as cytochrome c, were not reduced by vesicles loaded with the NADH-generating system. In contrast, right-side-out plasma membrane vesicles loaded with ascorbate supported the reduction of both ferric citrate and DCIP-sulfonate, suggesting that ascorbate also may serve as electron donor for transplasma membrane electron transport. Differences in substrate specificity and inhibitor sensitivity indicate that the electrons from ascorbate and NADH were channelled to external acceptors via different electron transport chains. Transplasma membrane electron transport constituted only about 10% of total plasma membrane electron transport activity, but should still be sufficient to be of physiological significance in, e.g. reduction of Fe3+ to Fe2+ for uptake.

  • 7.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Dept of Plant Physiology, Univ. of Lund, Lund, Sweden.
    Larsson, Christer
    Dept of Plant Physiology, Univ. of Lund, Lund, Sweden.
    Widell, Susanne
    Dept of Plant Physiology, Univ. of Lund, Lund, Sweden.
    Cytochromes of plant plasma membranes: Characterization by absorbance difference spectrophotometry and redox titration1989In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 76, no 2, p. 123-134Article in journal (Refereed)
    Abstract [en]

    The cytochrome composition of plasma membranes (PM) obtained by phase partitioning of microsomal fractions from spinach leaves (Spinacea oleracea L. cv. Medania), cauliflower inflorescences (Brassica oleracea L.), sugar beer leaves (Beta vulgaris L.) and barley (Hordeum vulgare L. cv. Kristina) roots and leaves was characterized by absorbance difference spectrophotometry at different reducing conditions at 20 and – 196°C, by redox titration, and by heme staining of polypeptide bands after lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE). The location of the α-bands in the difference spectra and the loss of heme after treatment with LDS indicated that predominantly cytochromes of the b-type were present in all species tested. The total concentration of cytochrome was ca 0.35 nmol (mg protein)−1. The main component (ca 70% of total) was completely reduced by ascorbate and partly by NADH and had a midpoint potential of ca 150 mV. At – 196°C, ascorbate reduction revealed a symmetrical α-band at ca 557 nm with PM from spinach leaves, cauliflower and sugar beet leaves, but with barley root and leaf PM ascorbate reduction resulted in an asymmetrical α-band (shoulder at 552, maximum at 559 nm). In the dithionite-reduced minus ascorbate-reduced spectrum at –196°C a split α-band (552 + 558 nm) was seen with PM from all species. This minor component had a midpoint potential of ca – 50 mV and is probably identical to cytochrome b5, the presence of which would explain the relatively high NADH-cytochrome c reductase activities observed with plant PM. With PM from cauliflower, CO-difference spectra indicated that cytochromes P-420 and P-450 were present at concentrations up to 0.06 and 0.03 nmol (mg protein)−1, respectively. Visualization of cytochromes by heme staining after LDS-PAGE was complicated by endogenous peroxidase activity and by loss of heme during solubilisation. A presumptive b-cytochrome (heme-stained band at 94 kDa) was only detected with barley leaf PM.

  • 8.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Physiology, University of Lund, Lund, Sweden.
    Larsson, Christer
    Department of Plant Physiology, University of Lund, Lund, Sweden.
    Widell, Susanne
    Department of Plant Physiology, University of Lund, Lund, Sweden.
    Localization of donor and acceptor sites of NADH dehydrogenase activities using inside-out and right-side-out plasma membrane vesicles from plants1988In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 239, no 1, p. 23-28Article in journal (Refereed)
    Abstract [en]

    Inside-out and right-side-out plasma membrane vesicles from sugar beet (Beta vulgaris L.) leaves, prepared by aqueous two-phase partitioning, were used to localize donor and acceptor sites and to determine substrate affinities for plasma membrane-bound NADH dehydrogenase activities. NADH-ferricyanide and NADH-cytochrome c reductase activities were approx. 30% latent with inside-out vesicles and about 80% latent with right-side-out vesicles, indicating that both donor and acceptor sites for these activities are located on the cytoplasmic surface of the plasma membrane, and that a possible transplasma membrane electron transport would constitute only a minor proportion of the total activity.

  • 9.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, University of Lund, Lund, Sweden.
    Laurent, Pascal
    Laboratoire de Biomembranes Vegetales, Unité de Recherche Associé 1180, Université Pierre et Marie Curie, Paris, France.
    Nakagawa, Hiroki
    Faculty of Horticulture, Chiba University, Matsudo, Chiba, Japan.
    Kader, Jean-Claude
    Laboratoire de Biomembranes Vegetales, Unité de Recherche Associé 1180, Université Pierre et Marie Curie, Paris, France.
    NADH-Ferricyanide Reductase of Leaf Plasma Membranes: Partial Purification and Immunological Relation to Potato Tuber Microsomal NADH-Ferricyanide Reductase and Spinach Leaf NADH-Nitrate Reductase1991In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 95, no 1, p. 6-13Article in journal (Refereed)
    Abstract [en]

    Plasma membranes obtained by two-phase partitioning of microsomal fractions from spinach (Spinacea oleracea L. cv Medania) and sugar beet leaves (Beta vulgaris L.) contained relatively high NADH-ferricyanide reductase and NADH-nitrate reductase (NR; EC 1.6.6.1) activities. Both of these activities were latent. To investigate whether these activities were due to the same enzyme, plasma membrane polypeptides were separated with SDS-PAGE and analyzed with immunoblotting methods. Antibodies raised against microsomal NADH-ferricyanide reductase (tentatively identified as NADH-cytochrome b5 reductase, EC 1.6.2.2), purified from potato (Solanum tuberosum L. cv Bintje) tuber microsomes, displayed one single band at 43 kilodaltons when reacted with spinach plasma membranes, whereas lgG produced against NR from spinach leaves gave a major band at 110 kilodaltons together with a few fainter bands of lower molecular mass. Immunoblotting analysis using inside-out and right-side-out plasma membrane vesicles strongly indicated that NR was not an integral protein but probably trapped inside the plasma membrane vesicles during homogenization. Proteins from spinach plasma membranes were solubilized with the zwitterionic detergent 3-[(3-cholamidopropyl) dimethylammonio] 1-propane-sulfonate and separated on a Mono Q anion exchange column at pH 5.6 with fast protein liquid chromatography. One major peak of NADH-ferricyanide reductase activity was found after separation. The peak fraction was enriched about 70-fold in this activity compared to the plasma membrane. When the peak fractions were analyzed with SDS-PAGE the NADH-ferricyanide reductase activity strongly correlated with a 43 kilodalton polypeptide which reacted with the antibodies against potato microsomal NADH-ferricyanide reductase. Thus, our data indicate that most, if not all, of the truly membrane-bound NADH-ferricyanide reductase activity of leaf plasma membranes is due to an enzyme very similar to potato tuber microsomal NADH-ferricyanide reductase (NADH-cytochrome b5 reductase).

  • 10.
    Askerlund, Per
    et al.
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research.
    Sommarin, M
    Calcium efflux transporters in higher plants1996In: Membranes: Specialized Functions in Plants / [ed] M. Smallwood, Oxford: Bios Scientific Publishers Ltd , 1996, p. 281-299Chapter in book (Refereed)
  • 11.
    Fredlund, Kenneth M.
    et al.
    Department of Plant Biology, Lund University, Lund, Sweden.
    Struglics, André
    Department of Plant Biology, Lund University, Lund, Sweden.
    Widell, Susanne
    Department of Plant Biology, Lund University, Lund, Sweden.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, Lund University, Lund, Sweden.
    Kader, Jean-Claude
    Laboratorie de Physiologie Cellulaire, Paris, France.
    Møller, Ian M.
    Department of Plant Biology, Lund University, Lund, Sweden.
    Comparison of the Stereospecificity and Immunoreactivity of NADH-Ferricyanide Reductases in Plant Membranes1994In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 106, no 3, p. 1103-1106Article in journal (Refereed)
    Abstract [en]

    The substrate stereospecificity of NADH-ferricyanide reductase activities in the inner mitochondrial membrane and peroxisomal membrane of potato (Solanum tuberosum L.) tubers, spinach (Spinacea oleracea L.) leaf plasma membrane, and red beetroot (Beta vulgaris L.) tonoplast were all specific for the [beta]-hydrogen of NADH, whereas the reductases in wheat root (Triticum aestivum L.) endoplasmic reticulum and potato tuber outer mitochondrial membrane were both [alpha]-hydrogen specific. In all isolated membrane fractions one or several polypeptides with an apparent size of 45 to 55 kD cross-reacted with antibodies raised against a microsomal NADH-ferricyanide reductase on western blots.

  • 12.
    Gabrielsson, Lovisa
    et al.
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine.
    Nilsson, Kristoffer
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine.
    Detektion av Trichomonas vaginalis samt Mycoplasma genitalium med multiplex realtids-PCR: En prevalensstudie i Jönköpings län2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The request for detection of Trichomonas vaginalis and Mycoplasma genitalium in Jönköping County is low compared to Chlamydia trachomatis and Neisseria gonorrhoeae. Both T. vaginalis and M. genitalium have been associated with Human Papilloma Virus (HPV) infection and can cause infections such as salpingitis, potentially resulting in infertility. The pathogens have also been described to increase the risk of HIV transmission. The aim of this study was to detect T. vaginalis and M. genitalium by real-time Polymerase Chain Reaction (PCR) to estimate the prevalence among individuals tested for C. trachomatis, N. gonorrhoeae and HPV in Jönköping County. In individuals above the age of 25 years, tested for C. trachomatis and N. gonorrhoeae, the prevalence was estimated to 5,5 % for M. genitalium and 0,13 % for T. vaginalis. In the same group the prevalence of C. trachomatis and N. gonorrhoeae was 4,5 % and 0,13 % respectively. The prevalence in individuals tested for HPV was estimated to 2,3 % for M. genitalium and 0,26 % for T. vaginalis. Relevance of a more frequent request for detection of M. genitalium was concluded and single pathogen detection was not deemed to be optimal. Multiplex analysis for detection of sexually transmitted pathogens is encouraged.

  • 13.
    Holmén, Jonathan
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Jansson, Andreas
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    A Kinetic Overview of the Receptors Involved in 1,25-Dihydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 Signaling: A Systems Biology Approach2009In: Critical Reviews in Eukaryotic Gene Expression, ISSN 1045-4403, E-ISSN 2162-6502, Vol. 19, no 3, p. 181-196Article, review/survey (Refereed)
    Abstract [en]

    The vitamin D endocrine system modulates an arsenal of important biological functions in more than 30 different tissues in short- and long-term perspectives. Two membrane receptors and one nuclear receptor are suggested to be involved in the vitamin D signaling system, but the function and physiological relevance of the receptors are debated. The complexity of the vitamin D endocrine system makes it necessary to combine experimental data with in silico simulations to get a holistic view of vitamin D-dependent regulation of tissue and cell physiology. This review focus on binding characteristics for the three putative vitamin D receptors and proposes a future systems biology approach including mathematical modeling that will be helpful together with experimental methods in depicting antitumoral and other biological effects promoted by the vitamin D endocrine system.

  • 14.
    Karlsson, Sandra
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Olausson, Josefin
    Högskolan i Skövde, Institutionen för vård och natur.
    Lundh, Dan
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sögård, Peter
    Högskolan i Skövde, Institutionen för vård och natur.
    Mandal, Abul
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Holmström, Kjell-Ove
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Stahel, Anette
    Högskolan i Skövde, Institutionen för vård och natur.
    Bengtsson, Jenny
    Högskolan i Skövde, Institutionen för vård och natur.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Vitamin D and prostate cancer: The role of membrane initiated signaling pathways in prostate cancer progression2010In: Journal of Steroid Biochemistry and Molecular Biology, ISSN 0960-0760, E-ISSN 1879-1220, Vol. 121, no 1-2, p. 413-416Article in journal (Refereed)
    Abstract [en]

    1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been demonstrated to mediate both genomic and non-genomic responses in prostate cancer (CaP) cells. Here, we give an overview of membrane initiated 1,25(OH)2D3 signaling in prostate cancer cell progression. The presence of PDIA3 was investigated and homologous modeling of the putative PDIA3 receptor complex was conducted. Furthermore, the cellular distribution of nVDR was analyzed. We could show that both nVDR and PDIA3 are expressed in the prostate cancer cell lines investigated. The homologous modeling of PDIA3 showed that the receptor complex exists in a trimer formation, which suggests for allosteric activity. Our findings support previous reports and suggest that 1,25(OH)2D3 is an important therapeutic agent in inhibiting prostate cancer progression. Furthermore, our data show that 1,25(OH)2D3 regulate prostate cell biology via multiple pathways and targeting specific pathways for 1,25(OH)2D3 might provide more effective therapies compared to the vitamin D therapies currently clinically tested.

  • 15. Kjellbom, Per
    et al.
    Larsson, Christer
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research.
    Schelin, Cecilia
    Widell, Susanne
    Cytochrome P-450/420 in plant plasma membranes: a possible component of the blue-light-reducible flavoprotein-cytochrome complex1985In: Photochemistry and Photobiology, ISSN 0031-8655, E-ISSN 1751-1097, Vol. 42, no 6, p. 779-783Article in journal (Refereed)
    Abstract [en]

    Carbon monoxide difference spectra and pyridine binding spectra indicate the presence of cytochrome P-450/420 in plasma membranes from cauliflower inflorescences. Mild lithium dodecylsulfate polyacrylamide gel electrophoresis shows only one heme staining band in the plasma membrane fraction at an apparent molecular weight of 93 kiloDalton. This band is suggested to be due to a cytochrome P-450/420 dimer, in view of the known molecular weights of animal cytochromes P-450/420. The plasma membrane-bound cytochrome P-450/420 is probably identical to the blue-light-reducible b-type cytochrome of plant plasma membranes, which has been inferred to have a role in photomorphogenesis.

  • 16.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Anderson, Deryk
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States / Kansas City University of Medicine and Biosciences, Kansas City, MO, United States.
    Smith, Nathan M.
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States.
    Nemere, Ilka
    Department of Nutrition and Food Sciences, Center for Integrated Biosystems, Utah State University, Logan, UT, United States / Department of Nutrition and Food Sciences, Utah State University, Logan, UT, United States.
    24,25-Dihydroxyvitamin D3 binds to catalase2006In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 97, no 6, p. 1259-1266Article in journal (Refereed)
    Abstract [en]

    There is increasing evidence that the vitamin D metabolite, 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) has endocrine actions. In the current work, we report that an endogenous binding protein for 24,25(OH)2D3 is catalase, based on sequence analysis of the isolated protein. An antibody (Ab 365) generated against equivalent protein recognized bovine catalase and a 64 kDa band in subcellular fractions of chick intestine. A commercially available anti-catalase antibody reduced specific [3H]24,25(OH)2D3 binding in subcellular fractions of chick intestine by greater than 65%, relative to the same fractions treated with an unrelated antibody (Ab 099). The same commercially available anti-catalase was able to block the inhibitory actions of 24,25(OH)2D3 on 32P uptake in isolated intestinal epithelial cell suspensions. We subsequently characterized binding of steroid to commercially available catalase, and found that between 0 and 5 nM of enzyme added to subcellular fraction P2 (20,000g, 10-min post-nuclear pellet) resulted in a linear increase in the amount of [3H]24,25(OH)2D3 specifically bound. Additional studies indicated that 25(OH)D3 was an effective competitor for binding, whereas 1,25(OH)2D3 only poorly displaced [3H]24,25(OH)2D3. Saturation analyses with added catalase yielded a physiologically relevant affinity constant (KD = 5.6 ± 2.7 nM) and a Bmax = 209 ± 34 fmols/mg protein, comparable to previous studies using purified basal lateral membranes or vesicular fractions. Moreover, in a study on subcellular fractions isolated from chickens of varying ages, we found that in females, both specific [3H]24,25(OH)2D3 binding and catalase activity increased from 7- to 58-week-old birds, whereas in males, elevated levels of both parameters were expressed in preparations of 7- and 58-week-old birds. The data suggest that signal transduction may occur through modulation of hydrogen peroxide production.

  • 17.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Hagberg, Malin
    Högskolan i Skövde, Institutionen för vård och natur.
    Nahren, Malek
    Högskolan i Skövde, Institutionen för vård och natur.
    Kjellberg, Charlotte
    Högskolan i Skövde, Institutionen för vård och natur.
    Senneberg, Edina
    Högskolan i Skövde, Institutionen för vård och natur.
    Tahmasebifar, Neda
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson, Viktoria
    Karolinska Institutet.
    Membrane Initiated Signaling by 1,25α-dihydroxyvitamin D3 in LNCaP Prostate Cancer Cells2008In: Hormonal Carcinogenesis V / [ed] Jonathan J. Li, Sara A. Li, Suresh Mohla, Henri Rochefort, Thierry Maudelonde, Springer, 2008, p. 573-579Chapter in book (Refereed)
    Abstract [en]

    Prostate cancer (PC) is one of the most common cancers among men, and vitamin D and its metabolites are candidates for prevention and therapy of this disease. The vitamin D metabolites, 1, 25-dihydroxyvitamin D3 (1,25D) and 25-hydroxyvitamin D3, decreases cellular proliferation and invasiveness, and stimulates differentiation of PC cells. However, the underlying mechanisms are not fully clarified, and there is evidence that some of these effects of the vitamin D system are mediated by specific membrane-associated receptors/binding proteins in addition to its nuclear receptor, suggesting multiple regulatory pathways. The aim of the present study was to examine the role of membrane initiated pathways mediating effects of 1,25D on cell invasiveness in LNCaP cells. Treatment with 1,25D evoked a dose-dependent activation of the JNK/SAPK MAPK signaling pathways within 10 min, demonstrating membrane initiated signaling of 1,25D in LNCaP cells. Furthermore, treatment with 1,25D decreased LNCaP cell invasiveness by approximately 20% after 48h. Using an inhibitor (SP600125) for the JNK/SAPK MAPK signaling pathway in combination with 1,25D on LNCaP cells, the inhibitory action of 1,25D on invasiveness was eliminated. In conclusion, 1,25D decrease invasiveness of LNCaP cells by interaction with a putative membrane associated receptor, which activate membrane, initiated signaling via the JNK/SAPK MAPK signaling pathway.

  • 18.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson, Viktoria
    Högskolan i Skövde, Institutionen för vård och natur.
    Karlsson, Sandra
    Högskolan i Skövde, Institutionen för vård och natur.
    Olausson, Josefin
    Högskolan i Skövde, Institutionen för vård och natur.
    Holmén, Jonathan
    Högskolan i Skövde, Institutionen för vård och natur.
    Hagberg, Malin
    Högskolan i Skövde, Institutionen för vård och natur.
    Rapid activation of JNK/SAPK in LNCaP prostate cancer cells by 1α,25-dihydroxyvitamin D3 is independent of PDIA3 (1,25-MARRS)2008In: Current Topics in Steroid Research, ISSN 0972-4788, Vol. 5, p. 17-24Article in journal (Refereed)
    Abstract [en]

    1α,25-dihydroxyvitamin D3 (1,25D3 ) is a highly potential anti-cancerous agent for prevention and treatment of prostate cancer, the most commonly diagnosed cancer type of males in western countries. A recent study by our laboratory, demonstrates that LNCaP cancer cells treated with 1,25D3, evoked dose-dependent activation of the JNK/SAPK MAPK signaling pathway within 10 minutes after hormone treatment, indicative of membrane-initiated steroid signaling (MISS) by 1,25D3. This confirms previous reports on intestinal-, chondrocyte- and osteoblast cells, where 1,25D3 operates through pharmacologically distinct nuclear-initiated mechanisms (NISS) and plasma membrane-initiated mechanisms. NISS is mediated via the vitamin D receptor (nVDR) and MISS is mediated through 1,25D3-MARRS (PDIA3, 1,25D3-membraneassociated rapid response steroid binding protein) or nVDR. The aims of the present study were to investigate the mechanisms of MISS evoked effects on alkaline phosphatase (ALP) and activation of the JNK/SAPK by 1,25D3, and the involvement of PDIA3 in 1,25D3 initiated activation of the JNK/SAPK signaling pathway. Furthermore, 1,25D3-treated LNCaP cells were transfected with siRNA against PDIA3 and phosphorylated JNK/SAPK was estimated by western analysis. Western analysis and ALP-assays demonstrated rapid activation of both JNK/SAPK as well as ALP. Silencing of PDIA3 did not affect 1,25D3 mediated activation of JNK/SAPK, suggesting that PDIA3 is not involved in the 1,25D3-initiated activation of the JNK/SAPK signaling pathway.

  • 19.
    Larsson, Dennis
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Jonas, Adele
    Högskolan i Skövde, Institutionen för vård och natur.
    Bergsten, Niklas
    Högskolan i Skövde, Institutionen för vård och natur.
    Ståhl, Fredrik
    University College of Borås, School of Health Sciences, Sweden.
    Karlsson, Sandra
    Högskolan i Skövde, Institutionen för vård och natur.
    Membrane Initiated Effects of1α,25-Dihydroxyvitamin D3 inProstate Cancer Cells: Effects on AP1 and CREB Mediated Transcription2012In: Current Frontiers and Perspectives in Cell Biology / [ed] Stevo Najman, InTech, 2012, p. 153-162Chapter in book (Refereed)
  • 20.
    Levefelt, Christer
    et al.
    School of Humanities and Informatics, University of Skövde, Skövde, Sweden.
    Lundh, Dan
    School of Humanities and Informatics, University of Skövde, Skövde, Sweden.
    A fold-recognition approach to loop modeling2006In: Journal of Molecular Modeling, ISSN 1610-2940, E-ISSN 0948-5023, Vol. 12, no 2, p. 125-139Article in journal (Refereed)
    Abstract [en]

    A novel approach is proposed for modeling loop regions in proteins. In this approach, a prerequisite sequence-structure alignment is examined for regions where the target sequence is not covered by the structural template. These regions, extended with a number of residues from adjacent stem regions, are submitted to fold recognition. The alignments produced by fold recognition are integrated into the initial alignment to create an alignment between the target sequence and several structures, where gaps in the main structural template are covered by local structural templates. This one-to-many (1:N) alignment is used to create a protein model by existing protein-modeling techniques. Several alternative approaches were evaluated using a set of ten proteins. One approach was selected and evaluated using another set of 31 proteins. The most promising result was for gap regions not located at the C-terminus or N-terminus of a protein, where the method produced an average RMSD 12% lower than the loop modeling provided with the program MODELLER. This improvement is shown to be statistically significant.

  • 21.
    Lundh, Dan
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Hedelin, Hans
    Skaraborgs Sjukhus, Skövde , Sweden.
    Jonsson, Karin
    Kärnsjukhuset, Skövde , Sweden.
    Gifford, Mervyn
    Högskolan i Skövde, Institutionen för vård och natur.
    Larsson, Dennis
    Högskolan i Skövde, Institutionen för vård och natur.
    Assessing chronic pelvic pain syndrome patients: Blood plasma factors and cortisol saliva2013In: Scandinavian journal of urology, ISSN 2168-1805, E-ISSN 2168-1813, Vol. 47, no 6, p. 521-528Article in journal (Refereed)
    Abstract [en]

    Objective:

    The aim of this study was to identify changes in inflammatory molecules in the blood (plasma) of patients with chronic prostatitis/chronic pelvic syndrome (CP/CPPS) compared with controls. Altered levels indicate a systemic component by possible involvement of the prostate and/or the inner pelvic floor musculature.

    Material and methods:

    In 32 patients with CP/CPPS and 37 controls, blood plasma levels of testosterone, macrophage migration inhibitory factor (MIF), tumour necrosis factor-alpha (TNF-alpha), TNF-beta, interleukin-2 (IL-2) and IL-1 beta were measured by enzyme-linked immunosorbent assay. Cortisol in saliva samples was measured in the morning and late evening. All participants answered a questionnaire regarding their health profile.

    Results:

    Significantly higher levels of MIF (p = 0.012) were detected in patients. The testosterone level was, contrary to other studies, little lower in patients (p = 0.014; age adjusted). When controls with health issues and patients with a parallel disease were excluded, the MIF and TNF-alpha levels were higher in the patients (p = 0.007, p = 0.016, respectively) than in controls, and the testosterone was slightly lower in patients (p = 0.047).

    Conclusions:

    The findings show an immune response extending to the circulatory system, in which MIF makes a significant contribution to CP/CPPS. This study also indicates TNF-alpha as a circulatory component when excluding subjects with concomitant diseases. Both MIF and TNF-alpha have previously been highlighted for other diseases related to chronic pain and here also for CP/CPPS. These results provide further insights into the immunological basis of CP/CPPS.

  • 22.
    Lundh, Dan
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Nahar, Noor
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Mandal, Abul
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Arsenic accumulation in plants - Outlining strategies for developing improved variety of crops for avoiding arsenic toxicity in foods2010In: Journal of biological systems, ISSN 0218-3390, Vol. 18, no 1, p. 223-241Article in journal (Refereed)
    Abstract [en]

    Contamination of food with arsenics is a potential health risk for both humans and animals in many regions of the world, especially in Asia. Arsenics can be accumulated in humans, animals and plants for a longer period and a long-term exposure of humans to arsenics results in severe damage of kidney, lever, heart etc. and many other vascular diseases. Arsenic contamination in human may also lead to development of cancer. In this paper we report our results on data mining approach (an in silico analysis based on searching of the existing genomic databases) for identification and characterization of genes that might be responsible for uptake, accumulation or metabolism of arsenics. For these in silico analyses we have involved the model plant Arabidopsis thaliana in our investigation. By employing a system biology model (a kinetic model) we have studied the molecular mechanisms of these processes in this plant. This model contains equations for uptake, metabolism and sequestration of different types of arsenic; As(V), As(III), MMAA and DMAA. The model was then implemented in the software XPP. The model was also validated against the data existing in the literatures. Based on the results of these in silico studies we have developed some strategies that can be used for reducing arsenic contents in different parts of the plant. Data mining experiments resulted in identification of two candidate genes (ACR2, arsenate reductase 2 and PCS1, phytochelatin synthase 1) that are involved either in uptake, transport or cellular localization of arsenic in A. thaliana. However, our system biology model revealed that by increasing the level of arsenate reductase together with an increased rate of arsenite sequestration in the vacuoles (by involving an arsenite efflux pump MRP1/2), it is possible to reduce the amount of arsenics in the shoots of A. thaliana to 11–12%.

  • 23.
    Malmström, Susanna
    et al.
    Molecular Biology Institute, Copenhagen University, Copenhagen, Denmark.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, Lund University, Lund, Sweden.
    Palmgren, Michael G.
    Molecular Biology Institute, Copenhagen University, Copenhagen, Denmark.
    A calmodulin-stimulated Ca2+-ATPase from plant vacuolar membranes with a putative regulatory domain at its N-terminus1997In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 400, no 3, p. 324-328Article in journal (Refereed)
    Abstract [en]

    A cDNA, BCA1, encoding a calmodulin-stimulated Ca2+-ATPase in the vacuolar membrane of cauliflower (Brassica oleracea) was isolated based on the sequence of tryptic peptides derived from the purified protein. The BCA1 cDNA shares sequence identity with animal plasma membrane Ca2+-ATPases and Arabidopsis thaliana ACA1, that encodes a putative Ca2+ pump in the chloroplast envelope. In contrast to the plasma membrane Ca2+-ATPases of animal cells, which have a calmodulin-binding domain situated in the carboxy-terminal end of the molecule, the calmodulin-binding domain of BCA1 is situated at the amino terminus of the enzyme.

  • 24. Møller, I. M.
    et al.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research.
    Widell, S.
    Electron transport constituents in the plant plasma membrane1991In: Oxidoreduction at the plasma membrane: Relation to growth and transport. Volume II: plants, Boca Raton: CRC Press , 1991, p. 35-59Chapter in book (Refereed)
  • 25. Palmgren, Michael Gjedde
    et al.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research. University of Lund.
    Fredrikson, Karin
    Widell, Susanne
    Sommarin, Marianne
    Larsson, Christer
    Sealed Inside-Out and Right-Side-Out Plasma Membrane Vesicles: Optimal Conditions for Formation and Separation1990In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 92, no 4, p. 871-880Article in journal (Refereed)
    Abstract [en]

    Plasma membrane preparations of high purity (about 95%) are easily obtained by partitioning in aqueous polymer two-phase systems. These preparations, however, mainly contain sealed right-side-out (apoplastic side out) vesicles. Part of these vesicles have been turned inside-out by freezing and thawing, and sealed inside-out and right-side-out vesicles subsequently separated by repeating the phase partition step. Increasing the KCI concentration in the freeze/thaw medium as well as increasing the number of freeze/thaw cycles significantly increased the yield of inside-out vesicles. At optimal conditions, 15 to 25% of total plasma membrane protein was recovered as inside-out vesicles, corresponding to 5 to 10 milligrams of protein from 500 grams of sugar beet (Beta vulgaris L.) leaves. Based on enzyme latency, trypsin inhibition of NADH-cytochrome c reductase, and H+ pumping capacity, a cross-contamination of about 20% between the two fractions of oppositely oriented vesicles was estimated. Thus, preparations containing about 80% inside-out and 80% right-side-out vesicles, respectively, were obtained. ATPase activity and H+ pumping were both completely inhibited by vanadate (Ki ≈ 10 micromolar), indicating that the fractions were completely free from nonplasma membrane ATPases. Furthermore, the polypeptide patterns of the two fractions were close to identical, which shows that the vesicles differed in sidedness only. Thus, preparations of both inside-out and right-side-out plasma membrane vesicles are now available. This permits studies on transport, signal transduction mechanisms, enzyme topology, etc., using plasma membrane vesicles of either orientation.

  • 26.
    Savill, Rachel
    Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine.
    Validering av realtids-PCR-metod för Herpes simplex- och Varicella-zoster virus2015Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 27.
    Sogaard, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Szekeres, Ferenc
    Karolinska Institutet.
    Garcia-Roves, Pablo M.
    Karolinska Institutet.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Chibalin, Alexander V.
    Karolinska Institutet.
    Zierath, Juleen R.
    Karolinska Institutet.
    Spatial Insulin Signalling in Isolated Skeletal Muscle Preparations2010In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 109, no 5, p. 943-949Article in journal (Refereed)
    Abstract [en]

    During in vitro incubation in the absence or presence of insulin, glycogen depletion occurs in the inner core of the muscle specimen, concomitant with increased staining of hypoxia-induced-factor-1-alpha and caspase-3, markers of hypoxia and apoptosis, respectively. The aim of this study was to determine whether insulin is able to diffuse across the entire muscle specimen in sufficient amounts to activate signalling cascades to promote glucose uptake and glycogenesis within isolated mouse skeletal muscle. Phosphoprotein multiplex assay on lysates from muscle preparation was performed to detect phosphorylation of insulin-receptor on Tyr1146, Akt on Ser473 and glycogen-synthases-kinase-3 on Ser21/Ser9. To address the spatial resolution of insulin signalling, immunohistochemistry studies on cryosections were performed. Our results provide evidence to suggest that during the in vitro incubation, insulin sufficiently diffuses into the centre of tubular mouse muscles to promote phosphorylation of these signalling events. Interestingly, increased insulin signalling was observed in the core of the incubated muscle specimens, correlating with the location of oxidative fibres. In conclusion, insulin action was not restricted due to insufficient diffusion of the hormone during in vitro incubation in either extensor digitorum longus or soleus muscles from mouse under the specific experimental settings employed in this study. Hence, we suggest that the glycogen depleted core as earlier observed is not due to insufficient insulin action.

  • 28. Stangeland, B.
    et al.
    Fuglsang, A. T.
    Malmström, S.
    Axelsen, K. B.
    Baunsgaard, L.
    Lanfermeijer, F. C.
    Venema, K.
    Okkels, F. T.
    Askerlund, Per
    Jönköping University, School of Education and Communication, HLK, Disciplinary Research.
    Palmgren, M. G.
    P-type H(+)- and Ca(2+)-ATPases in plant cells1997In: Na/K-ATPase and related transport ATPases: structure, mechanism, and regulation / [ed] Luis A. Beaugé, David C. Gadsby, and Patricio J. Garrahan, New York: New York Academy of Sciences , 1997, Vol. 834, p. 77-87Chapter in book (Refereed)
  • 29.
    Sögaard, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Szekeres, Ferenc
    Department of Molecular Medicine and Surgery, Section og Integrative Physiology, Karolinska Institutet.
    Holmström, Maria
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Harlén, Mikael
    Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Garcia-Roves, Pablo
    Section of Integrative Physiology, Department of Physiology and Pharmacology, Karolinska Institutet.
    Chibalin, Alexander V.
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Effects of fibre type and diffusion distance on mouse skeletal muscle glycogen content in vitro2009In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 107, no 6, p. 1189-1197Article in journal (Refereed)
    Abstract [en]

    In vitro incubation of isolated rodent skeletal muscle is a widely used procedure in metabolic research. One concern with this method is the development of an anoxic state during the incubation period that can cause muscle glycogen depletion. Our aim was to investigate whether in vitro incubation conditions influence glycogen concentration in glycolytic extensor digitorum longus (EDL) and oxidative soleus mouse muscle. Quantitative immunohistochemistry was applied to assess glycogen content in incubated skeletal muscle. Glycogen concentration was depleted, independent of insulin-stimulation in the incubated skeletal muscle. The extent of glycogen depletion was correlated with the oxidative fibre distribution and with the induction of hypoxia-induced-factor-1-alpha. Insulin exposure partially prevented glycogen depletion in soleus, but not in EDL muscle, providing evidence that glucose diffusion is not a limiting step to maintain glycogen content. Our results provide evidence to suggest that the anoxic milieu and the intrinsic characteristics of the skeletal muscle fibre type play a major role in inducing glycogen depletion in during in vitro incubations.

  • 30.
    Wadskog, Ingrid
    Jönköping University.
    Functional studies of the Sro yeast homologues of the Drosophila lethal (2) giant larvae tumor suppressor genes2003Doctoral thesis, monograph (Other scientific)
1 - 30 of 30
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