This paper investigates the conditions for the assimilation of information systems (IS) and information technology (IT) in organizations and the influence of various actors in the organization and eBusiness context. To do so it draws on the literature on assimilation gap, sociomateriality and infrastructure together with a study of implementation and use of information and communication technology in and among organizations in a Swedish region. There were substantial investments in web infrastructure made on a regional level and the focus of this study is how the investments were transformed and assimilated in practices, relations and communication. Based on the empirical data from the case study, the paper extends the assimilation process into interplay among actors in organizations contexts. It describes organizations’ strategies for coping with their needs for information and the actors in these processes. Two categories of actors are identified, sensemaking and sensegiving actors, as most important in assimilation of IS/IT in organizations. A sociomaterial perspective gives guidance and a better understanding of the assimilation process in terms of knowledge and interpretative frames, and how assimilation involves identity construction and negotiations among sensemaking and sensegiving actors. The contribution of this paper is a better understanding of the context of assimilation and adaptation of IT in organizations’ business processes, and steps to be taken to improve readiness.