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Efficient expansion of cryopreserved CD4+ CD25+CD127lo/-cells in Type 1 diabetes
Linköping University.
Jönköping University, School of Health and Welfare, HHJ, Department of Clinical Diagnostics. Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
2011 (English)In: Results in Immunology, E-ISSN 2211-2839, Vol. 1, no 1, p. 36-44Article in journal (Refereed) Published
Abstract [en]

Increased attention has been drawn to the important role played by regulatory T-cells (Treg) in immune homoeostasis. However, the small numbers of Tregs make them elusive to study. We investigated the cryostability of Tregs and whether they can be expanded from cryopreserved peripheral blood mononuclear cells (PBMCs). Further, to elucidate if there is a difference in ex-vivo frequency or in vitro expansion of Tregs among T1D children (n=9), high-risk (n=7) and healthy (n=10) individuals, Tregs defined as CD4+CD25+CD127lo/− were isolated from cryopreserved PBMCs.

Cryopreserved PBMCs maintained a stable expression of Treg-markers. Tregs were efficiently expanded in vitro from all donors and Tregs from T1D children acquired higher FOXP3 expression compared to healthy subjects. T1D children had a significantly lower percentage of Tregs among CD4+ T-cells and also lower Treg to CD4+CD25 cell ratios compared to healthy individuals.

Place, publisher, year, edition, pages
2011. Vol. 1, no 1, p. 36-44
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Other Medical Sciences
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URN: urn:nbn:se:hj:diva-16091DOI: 10.1016/j.rinim.2011.08.001PubMedID: 24371551OAI: oai:DiVA.org:hj-16091DiVA, id: diva2:441700
Available from: 2011-09-19 Created: 2011-09-19 Last updated: 2024-01-18Bibliographically approved

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Faresjö, Maria

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