Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Efficient expansion of cryopreserved CD4+ CD25+CD127lo/-cells in Type 1 diabetes
Linköping University.
Jönköping University, School of Health Science, HHJ, Dep. of Natural Science and Biomedicine. Jönköping University, School of Health Science, HHJ. Biomedical Platform.
2011 (English)In: Results in Immunology, ISSN 2211-2839, Vol. 1, no 1, 36-44 p.Article in journal (Refereed) Published
Abstract [en]

Increased attention has been drawn to the important role played by regulatory T-cells (Treg) in immune homoeostasis. However, the small numbers of Tregs make them elusive to study. We investigated the cryostability of Tregs and whether they can be expanded from cryopreserved peripheral blood mononuclear cells (PBMCs). Further, to elucidate if there is a difference in ex-vivo frequency or in vitro expansion of Tregs among T1D children (n=9), high-risk (n=7) and healthy (n=10) individuals, Tregs defined as CD4+CD25+CD127lo/− were isolated from cryopreserved PBMCs.

Cryopreserved PBMCs maintained a stable expression of Treg-markers. Tregs were efficiently expanded in vitro from all donors and Tregs from T1D children acquired higher FOXP3 expression compared to healthy subjects. T1D children had a significantly lower percentage of Tregs among CD4+ T-cells and also lower Treg to CD4+CD25 cell ratios compared to healthy individuals.

Place, publisher, year, edition, pages
2011. Vol. 1, no 1, 36-44 p.
National Category
Other Medical Sciences
Identifiers
URN: urn:nbn:se:hj:diva-16091DOI: 10.1016/j.rinim.2011.08.001OAI: oai:DiVA.org:hj-16091DiVA: diva2:441700
Available from: 2011-09-19 Created: 2011-09-19 Last updated: 2011-09-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Faresjö, Maria
By organisation
HHJ, Dep. of Natural Science and BiomedicineHHJ. Biomedical Platform
Other Medical Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 250 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf