Virtual learning environments have in recent years become an important tool in higher education, in distance learning as well as on campus. This study analyzed factors influencing acceptance of virtual learning environments among academic staff and students in blended learning environments. The study was performed as a comparative, explanatory case study at three universities providing master education of public health in Sweden, Norway and Lithuania. The findings of the case study showed that national culture was powerful in influencing acceptance of virtual learning environments, positively as well as negatively. High degrees of performance expectancy and social influence affected acceptance of virtual learning environments positively. The high degree of social influence was hypothesized to be transferred by the values of the national culture. The high degree of acceptance of virtual learning environments in Lithuania was hypothesized to be due to high degrees of the cultural values of power distance and uncertainty-avoidance.