Not much is known about the impact of national culture on perceptions of e-learning. The aim of the study was to compare perceptions of e-learning implementation among university teachers at a Swedish and an Argentinean university. Hofstede’s (2001) four dimensions of national culture were used to characterise the national cultures of Sweden and Argentina: power distance, uncertainty avoidance, individualism – collectivism, and masculinity – femininity. 159 Swedish and 110 Argentinean university teachers answered a survey questionnaire on purposes of using e-learning in university education, and driving factors and barriers towards e-learning implementation. The responses to the questionnaire were mainly similar. The only difference in responses due to national culture was that Argentinean university teachers stressed lack of incentives and appreciation as a more serious barrier to e-learning implementation than the Swedish respondents. This difference might be due to the more masculine culture of Argentina, stressing recognition and rewards more than the feminine Swedish culture.