Currently, little is known if and how national culture shapes e-learning. In this study, attitudes, purposes, driving factors and barriers of e-learning use at an Argentinean and a Swedish university was compared. A survey questionnaire was distributed among teachers at both universities. Data was analysed to discern similarities and differences in responses from Argentinean and Swedish teachers from the four cultural dimensions developed by Hofstede; power distance, uncertainty avoidance, individualism – collectivism, and masculinity – femininity. The main conclusion of the study was that the similarities of responses from Swedish and Argentinean teachers were more common than differences. However, some differences emanating from national culture could be discerned. Argentinean teachers stated communication with students and active student participation as more important driving factors than Swedish teachers. Lack of incentives and appreciation was stated as more important barriers among the Argentinean teachers. These findings are hypothesised to be due to the more collectivist and masculine culture of Argentina.