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Transmembrane Electron Transport in Plasma Membrane Vesicles Loaded with an NADH-Generating System or Ascorbate
Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Biochemistry, University of Lund, Lund, Sweden.ORCID iD: 0000-0003-0117-2974
Department of Plant Biochemistry, University of Lund, Lund, Sweden.
1991 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 96, no 4, 1178-1184 p.Article in journal (Refereed) Published
Abstract [en]

Sugar beet (Beta vulgaris L.) leaf plasma membrane vesicles were loaded with an NADH-generating system (or with ascorbate) and were tested spectrophotometrically for their ability to reduce external, membrane-impermeable electron acceptors. Either alcohol dehydrogenase plus NAD+ or 100 millimolar ascorbate was included in the homogenization medium, and right-side-out (apoplastic side-out) plasma membrane vesicles were subsequently prepared using two-phase partitioning. Addition of ethanol to plasma membrane vesicles loaded with the NADH-generating system led to a production of NADH inside the vesicles which could be recorded at 340 nanometers. This system was able to reduce 2,6-dichlorophenolindophenol-3′-sulfonate (DCIP-sulfonate), a strongly hydrophilic electron acceptor. The reduction of DCIP-sulfonate was stimulated severalfold by the K+ ionophore valinomycin, included to abolish membrane potential (outside negative) generated by electrogenic transmembrane electron flow. Fe3+-chelates, such as ferricyanide and ferric citrate, as well as cytochrome c, were not reduced by vesicles loaded with the NADH-generating system. In contrast, right-side-out plasma membrane vesicles loaded with ascorbate supported the reduction of both ferric citrate and DCIP-sulfonate, suggesting that ascorbate also may serve as electron donor for transplasma membrane electron transport. Differences in substrate specificity and inhibitor sensitivity indicate that the electrons from ascorbate and NADH were channelled to external acceptors via different electron transport chains. Transplasma membrane electron transport constituted only about 10% of total plasma membrane electron transport activity, but should still be sufficient to be of physiological significance in, e.g. reduction of Fe3+ to Fe2+ for uptake.

Place, publisher, year, edition, pages
1991. Vol. 96, no 4, 1178-1184 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:hj:diva-2179PubMedID: 16668317OAI: oai:DiVA.org:hj-2179DiVA: diva2:32999
Available from: 2007-10-17 Created: 2007-10-17 Last updated: 2017-02-13Bibliographically approved

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