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The tongue biofilm metatranscriptome identifies metabolic pathways associated with the presence or absence of halitosis
Genomics & Health Department, FISABIO Institute, Valencia, Spain.
Genomics & Health Department, FISABIO Institute, Valencia, Spain.
Department of Stomatology, University of Valencia, Valencia, Spain.
Department of Stomatology, University of Valencia, Valencia, Spain.
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2022 (English)In: npj Biofilms and Microbiomes, E-ISSN 2055-5008, Vol. 8, no 1, article id 100Article in journal (Refereed) Published
Abstract [en]

Intra-oral halitosis usually results from the production of volatile sulfur compounds, such as methyl mercaptan and hydrogen sulfide, by the tongue microbiota. There are currently no reports on the microbial gene-expression profiles of the tongue microbiota in halitosis. In this study, we performed RNAseq of tongue coating samples from individuals with and without halitosis. The activity of Streptococcus (including S. parasanguinis), Veillonella (including V. dispar) and Rothia (including R. mucilaginosa) was associated with halitosis-free individuals while Prevotella (including P. shahi), Fusobacterium (including F. nucleatum) and Leptotrichia were associated with halitosis. Interestingly, the metatranscriptome of patients that only had halitosis levels of methyl mercaptan was similar to that of halitosis-free individuals. Finally, gene expression profiles showed a significant over-expression of genes involved in L-cysteine and L-homocysteine synthesis, as well as nitrate reduction genes, in halitosis-free individuals and an over-expression of genes responsible for cysteine degradation into hydrogen sulfide in halitosis patients.

Place, publisher, year, edition, pages
Nature Publishing Group, 2022. Vol. 8, no 1, article id 100
Keywords [en]
cysteine, homocysteine, methyl mercaptan, nitrate, thiol, unclassified drug, hydrogen sulfide, methanethiol, thiol derivative, adult, aged, amino acid synthesis, Article, biofilm, coating thickness, controlled study, female, Fusobacterium nucleatum, gene overexpression, halitosis, human, Leptotrichia, major clinical study, male, metabolism, metatranscriptomics, microbial community, nonhuman, Prevotella, Prevotella shahi, RNA sequencing, Rothia mucilaginosa, Streptococcus parasanguinis, tongue, Veillonella, Veillonella dispar, microbiology, Biofilms, Humans, Metabolic Networks and Pathways, Sulfhydryl Compounds
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Dentistry
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URN: urn:nbn:se:hj:diva-59282DOI: 10.1038/s41522-022-00364-2ISI: 000900878000001PubMedID: 36535943Scopus ID: 2-s2.0-85144285833Local ID: HOA;intsam;59282OAI: oai:DiVA.org:hj-59282DiVA, id: diva2:1723430
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EU, European Research CouncilEuropean Social Fund (ESF)Available from: 2023-01-03 Created: 2023-01-03 Last updated: 2023-01-19Bibliographically approved

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Mira, Alex

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