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Detektion och kvantifiering av två olika isoformer av PDIA3 i prostatacancerceller
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2019 (Swedish)Independent thesis Basic level (professional degree), 180 HE creditsStudent thesisAlternative title
Detection and quantification of two different isoforms of PDIA3 in prostate cancer cells (English)
Abstract [sv]

Det finns ett behov av fler specifika biomarkörer kopplade till prostatacancer, vilka skulle öka behandlingsmöjligheter vid tidig diagnos. Olika isoformer av protein disulfide isomerase family A, member 3 (PDIA3) är associerade med maligna stadier av prostatacancer och kan därför potentiellt användas som biomarkörer. Syftet med studien var att detektera, kvantifiera och undersöka genuttryck av den proteinkodande isoformen PDIA3-201 och en nyupptäckt isoform av PDIA3 (PDIA3-Novel) i prostatacancerceller i varierande stadier. Uttrycket undersöktes med droplet digital Polymerase Chain Reaction (ddPCR) i normala prostataceller och i prostatacancerceller (PNT2, DU145, PC3 och LNCaP). Variation observerades mellan replikat och olika spädningar. Förhållanden av koncentrationer mellan PDIA3-Novel och PDIA3-201 skiljde sig både inom och mellan dem olika cellinjerna. Uttrycket av PDIA3-Novel var högre i PNT2 och PC3 jämfört med DU145. Uttrycket av PDIA3-Novel var högre i metastatiskt stadium av prostatacancer (LNCaP) men bristerna i studien minskar tillförlitligheten av resultatet. Resultatet från denna studie indikerar dock att PDIA3-Novel kan användas som biomarkör för prostatacancer och ge information som gynnar behandling. Fler försök i större skala behöver utföras som inkluderar patientprover och fler cellinjer.

Abstract [en]

There is a need for more specific biomarkers related to prostate cancer, which would increase treatment options. Different isoforms of protein disulfide isomerase family A, member 3 (PDIA3) are associated with prostate cancer and can potentially be used as biomarkers. The aims of this study were to detect, quantify and examine the gene expression of the protein coding isoform PDIA3-201 and a newly discovered isoform of PDIA3 (PDIA3-Novel) in prostate cancer cells in varying stages. The expression was analysed using droplet digital Polymerase Chain Reaction (ddPCR) in normal prostate cells and in prostate cancer cell lines (PNT2, DU145, PC3 and LNCaP). Variation within replicates and between different dilutions were observed. The ratio of concentrations between PDIA3-Novel and PDIA3-201 differed both within and between the cell lines. The expression of PDIA3-Novel was higher in PNT2 and PC3 compared to DU145. The expression of PDIA3-Novel was higher in the metastatic stage of prostate cancer (LNCaP), but the flaws in the study reduces the reliability of the results. The results of this study indicate that PDIA3-Novel can be used as a biomarker for prostate cancer and provide information beneficial for treatment. More experiments on a larger scale needs to be performed.

Place, publisher, year, edition, pages
2019. , p. 33
Keywords [en]
1, 25(OH)2D3, 25(OH)D, ddPCR, PSA, VDR
Keywords [sv]
1, 25(OH)2D3, 25(OH)D, ddPCR, PSA, VDR
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-45216ISRN: JU-HHJ-BLA-1-20190090OAI: oai:DiVA.org:hj-45216DiVA, id: diva2:1333148
Subject / course
HHJ, Biomedical Laboratory Science; HHJ, Biomedical Laboratory Science
Presentation
2019-06-04, Ga1031 Hälsohögskolan, Barnarpsgatan 39, Hus G, Jönköping, Jönköping, 11:00 (Swedish)
Supervisors
Examiners
Available from: 2019-07-03 Created: 2019-06-30 Last updated: 2019-07-03Bibliographically approved

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