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Ättiksyrans påverkan på mesotelcellers morfologi vid tvätt av exsudat
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2019 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

En vanlig orsak till otillfredsställande cytologiska preparat är kontamination av blod. På Cytologilaboratoriet, Länssjukhuset Ryhov tvättas blodiga gynekologiska preparat i Cytolyt® solution och ättiksyra för att reducera kontaminationer. Syftet med studien var att studera den potentiella påverkan tvätt med Cytolyt® solution och ättiksyra har på mesotelcellers morfologi. I studien ingick 60 exsudat av pleuravätska och ascites som analyserades med konventionell cytologisk metod. Utstryk utfördes och analyserades utan tvätt (kontroller) och efter tvätt i olika tider varpå preparaten bedömdes med avseende på mesotelcellers morfologi. Av kontrollerna hade 53% en välbevarad cytoplasma men endast 5.8% av de tvättade proverna. Cirka 87% av kontrollerna och 46% av de tvättade proverna hade en välbevarad och distinkt cellkärna. Morfologiska förändringar hos mesotelceller kunde påvisas efter tvätt i Cytolyt® solution och ättiksyra men förändringarna kunde inte kopplas till eller misstolkas som patologiska förändringar. 

Abstract [en]

A common reason for unsatisfactory cytological smears is contamination of blood. Blood contaminated gynaecological samples are washed with Cytolyt® solution and acetic acid at the Cytological Laboratory, County Hospital Ryhov to reduce contaminations. The objective of this study was to determine the potential effect of Cytolyt® solution and acetic acid on the morphology of mesothelial cells. In this study 60 samples of pleural effusions and ascites was analysed with conventional cytological method. Cytological smears were prepared without wash (controls) and with wash for different durations. The morphology of mesothelial cells in the samples were then examined. Out of the controls 53% had a wellpreserved cytoplasm while this distribution decreased to 5.8% amongst the washed smears. About 87% of the controls and 46% of the washed smears had a preserved and distinct nucleus. Changes in morphology could be seen in mesothelial cells after wash with Cytolyt® solution and acetic acid. However, the changes could not be associated with disease. Washing exudates with Cytolyt® solution and acetic acid can alter morphological changes in mesothelial cells although the changes seen cannot be mistaken for pathological changes. 

Place, publisher, year, edition, pages
2019. , p. 13
Keywords [sv]
Pleuravätska, ascites, konventionell cytologi, ättiksyra, cytologi
National Category
Other Medical Sciences not elsewhere specified
Identifiers
URN: urn:nbn:se:hj:diva-44649ISRN: JU-HHJ-BLA-1-20190085OAI: oai:DiVA.org:hj-44649DiVA, id: diva2:1326884
External cooperation
Region Jönköpings Län
Subject / course
HHJ, Biomedical Laboratory Science
Presentation
2019-06-03, Konferensrum Pipetten, Länssjukhuset Ryhov, Sjukhusgatan, Jönköping, 10:00 (Swedish)
Supervisors
Examiners
Available from: 2019-06-26 Created: 2019-06-18 Last updated: 2019-06-26Bibliographically approved

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Ättiksyrans påverkan på mesotelcellers morfologi vid tvätt av exsudat(438 kB)2 downloads
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CiteExportLink to record
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Citation style
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