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Prevalens av Anaplasma phagocytophilum och Candidatus Neoehrlichia mikurensis i Ixodes ricinus fästingar insamlade i Nordnorge
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2019 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Prevalence of Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis in Ixodes ricinus ticks collected in northern Norway (English)
Abstract [sv]

Den ökande spridningen av fästingburna sjukdomar är ett växande problem i Europa. Anaplasma phagocytophilum och Candidatus Neoehrlichia mikurensis är två humanpatogena bakterier som fästingen Ixodes ricinus agerar vektor för. Klimatförändringar har medfört att I. ricinus expanderat till allt nordligare breddgrader och därmed även fästingburna sjukdomar. Syftet med studien var att undersöka prevalensen av A. phagocytophilum samt Ca. N. mikurensis genom realtids-PCR på I. ricinus fästingar insamlade i Nordnorge. Materialet har tidigare analyserats för Borrelia burgdorferi sensu lato samt Rickettsia spp. Totalt analyserades 557 fästingar varav 354 var fältinsamlade och 203 var plockade från djur. Vid positivt fynd verifierades resultatet med Sangersekvensering. Resultaten från studien uppvisar en prevalens på 2,3% för A. phagocytophilum och 0,4% för Ca. N. mikurensis. Dessa resultat är lägre för både A. phagocytophilum och Ca. N. mikurensis jämfört med tidigare studier utförda på liknande breddgrader. Co-infektion med Borrelia afzelii detekterades för båda bakterierna. För att erhålla en djupare förståelse för spridningen och prevalensen för de båda bakteriearterna bör fler och upprepande studier utföras på både tama och vilda djur samt värdsökande fästingar i området.

Abstract [en]

The increasing spread of tick-borne diseases is a growing problem in Europe. The Ixodes ricinus tick is the vector of two human pathogens: Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis. Due to climate changes the I. ricinus tick is expanding further north and with it, the tick-borne diseases. The aim of this study was to determine the prevalence of A. phagocytophilum and Ca. N. mikurensis in I. ricinus ticks collected in the northern part of Norway, using real-time PCR. The material was previously analyzed for Borrelia burgdorferi sensu lato and Rickettsia spp. A total of 557 ticks were analyzed where 354 were questing ticks and 203 were collected from pets. Positive result was confirmed by Sanger sequencing. The result showed a prevalence of 2.3% for A. phagocytophilum and 0.4% for Ca. N. mikurensis. Compared to previous studies in the same area both A. phagocytophilum and Ca. N. mikurensis showed lower prevalence. Co-infection with Borrelia afzelii was detected for both bacteria species. Further studies of the prevalence for both bacteria needs to be done continuously, not only in questing ticks but also in ticks collected from domestic and wild animals, to get a deeper understanding of how these tick-borne bacteria are spreading.

Place, publisher, year, edition, pages
2019. , p. 20
Keywords [en]
Neoehrlichiosis, human granulocytic anaplasmosis, TaqMan, SYBR green, tick-borne pathogens
Keywords [sv]
Neoehrlichios, human granulocytisk anaplasmos, TaqMan, SYBR green, fästingburna patogener
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-44596ISRN: JU-HHJ-BLA-1-20190084OAI: oai:DiVA.org:hj-44596DiVA, id: diva2:1326150
Subject / course
HHJ, Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2019-06-26 Created: 2019-06-17 Last updated: 2019-06-26Bibliographically approved

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CiteExportLink to record
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