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Underdiagnostisering av tarmparasiter hos patienter med diarrébesvär
Jönköping University, School of Health and Welfare, HHJ. Biomedical Platform.
2017 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Underdiagnosis of intestinal parasites in patients with diarrhea (English)
Abstract [en]

Underdiagnosis of intestinal parasites in patients with diarrhea

A compilation from the Swedish public health authority indicates that infections caused by Cryptosporidium spp. increased in Sweden from 47 cases in 2004 to 594 cases in 2016 and Giardia intestinalis causes around 1300 infections per year. The primary aim of this study was to investigate the prevalence of parasites in patients with diarrhea. Furthermore, the study investigated whether samples taken with E-swab could be analyzed with real-time polymerase chain reaction (PCR) for detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica/dispar and G. intestinalis rather than Sodium acetate-acetic acid-formaline fixative (SAF-fixative). Prevalence of parasites in fecal samples was collected from 200 samples from patients with bacterial issue ordered. For evaluation of E-swab, 22 frozen, unfixed samples that were positive for intestinal parasites was used. Twelve positive E-swab samples was used as comparative positive controls. This was analyzed using real-time PCR. Bacteria was counted for 9.5% of the infections whilst parasites counted for 14% of the infections. The conclusion was that E-swab could replace SAF-fixative in the diagnosis of intestinal parasites and that there is that an underdiagnosis of intestinal parasites.

Keywords: Cryptosporidium spp, Dientamoeba fragilis, Entamoeba histolytica, Entamoeba dispar, Giardia intestinalis, real-time PCR, E-swab, prevalence.

Place, publisher, year, edition, pages
2017. , 21 p.
Keyword [sv]
Cryptosporidium spp, Dientamoeba fragilis, Entamoeba histolytica, Entamoeba dispar, Giardia intestinalis, realtids-PCR, E-swab, prevalens
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-36532ISRN: JU-HHJ-BLA-1-20170052OAI: oai:DiVA.org:hj-36532DiVA: diva2:1118081
Subject / course
HHJ, Biomedical Laboratory Science
Presentation
2017-06-07, pipetten, Sjukhusgatan, 553 05, Jönköping, 08:30 (Swedish)
Supervisors
Examiners
Available from: 2017-06-30 Created: 2017-06-29 Last updated: 2017-06-30Bibliographically approved

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Andersson, SaraLidman, Emma
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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More styles
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  • de-DE
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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Output format
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