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Aspartat aminotransferas enzymaktivitet mätt i Advia 1800 och Sigma Aldrich AST AAK: En jämförande analys
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2017 (Swedish)Independent thesis Basic level (degree of Bachelor), 180 HE creditsStudent thesisAlternative title
Aspartate aminotransferase enzyme activity measured in Advia 1800 and Sigma Aldrich AST AAK : A comparative analysis (English)
Abstract [sv]

När leverceller drabbas av sjukdom eller cellsönderfall läcker intracellulära ämnen ut i blodet. Ämnena kan mätas på olika sätt. För att läkare ska kunna ta korrekta beslut baserade på bland annat laboratorieresultat, är det viktigt att utvärdera instrument med avseende både på reliabilitet och likvärdighet. Syftet var att jämföra Advia 1800 med Sigma Aldrichs Aspartat aminotransferas (AST) activity assay kit (AAK) avseende enzymaktivitet. I Advia 1800 analyserades 40 prover med patientserum och frystes sedan in i -20° C för att förhindra fortsatt enzymnedbrytning. Analys utfördes sedan med AST AAK med upptinade serumprov. Beräkningarna av resultaten visar att de båda metoderna har en korrelationskoefficient på 0,104 och är därmed inte jämförbara. P-värdet var <0,05

Abstract [en]

When liver cells become subject to disease or disintegration intracellular substances will leak into the blood. These substances can be measured in different ways. For physicians to make adequate decisions based upon laboratory results, among others, it is important to evaluate instruments for both reliability and equivalence. The aim was to compare Advia 1800 with Sigma Aldrich´s Aspartate aminotransferase (AST) activity assay kit (AAK) concerning enzyme activity. In Advia 1800, 40 samples of patient serum where analyzed and then frozen at -20° C to prevent further enzyme degradation. Analysis was then performed with AST AAK on thawed samples. Results show a correlation coefficient of 0,104 and the two methods are not comparable. The p-value was <0,05

Place, publisher, year, edition, pages
2017. , p. 16
Keywords [en]
Livercell damage, cirrhosis, fibrosis, cell disintegration, intracellular
Keywords [sv]
Levercellskador, cirros, fibros, cellsönderfall, intracellulär
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-36219ISRN: JU-HHJ-BLA-1-20170050OAI: oai:DiVA.org:hj-36219DiVA, id: diva2:1111241
External cooperation
Länssjukhuset Ryhov
Subject / course
HHJ, Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2017-06-28 Created: 2017-06-18 Last updated: 2017-06-28Bibliographically approved

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Biomedical Laboratory Science/Technology

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CiteExportLink to record
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Citation style
  • apa
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