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Utvärdering av Copan EswabTM för viabilitet av bakterier
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2017 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Evaluation of Copan Eswab™ for viability of bacteria (English)
Abstract [sv]

Bakterier har alltid haft en stor inverkan på mänskligheten. För att diagnostisera bakteriella sjukdomar och behandla dem krävs identifiering av bakterien eller bakteriens relevanta egenskaper. Transportmedium har utvecklats för att hålla bakterierna vid liv från provtagning till analys. Syftet med studien var att utvärdera bakteriers viabilitet i det vätskebaserade mediet Copan Eswab jämfört med kolmedium (Copan swab). Bakterierna som ingick i studien var Campylobacter jejuni, Streptococcus pneumoniae, Haemophilus influenzae, Niesseria gonorrhoeae och Fusobacterium nucleatum. Förutom jämförande mellan medierna genomfördes en jämförelse mellan Eswab i kyl och i rumstemperatur. Resultaten för H. influenzae (n=9) och N. gonorrhoeae (n=9) visade att Eswab gav lika många eller fler överlevande bakterier. Gällande F. nucleatum (n=9) visade resultaten att fler överlevde i Copan swab (Copanpinnar) de första 28 timmarna, men även att bakterien inte klarar mer än 28 timmar i rumstemperatur. Gällande S. pneumoniae (n=9) och C. jejuni (n=9) gav båda opålitliga svar. Ytterligare mätpunkter och studier krävs för att erhålla mer pålitliga resultat gällande hur länge bakterierna överlever i Eswab.

Abstract [en]

Bacteria have always had a great influence on mankind. To diagnose any bacterial disease and treat it it’s necessary to identify the bacteria or any relevant attributes. Different types of specimen transport have been developed to keep the bacteria alive from sampling until the analysis is performed. The purpose of the study was to evaluate the viability of bacteria in the fluid-based media Copan EswabTM compared with charcoal medium (Copan swab). Bacteria included in the study were: Campylobacter jejuni, Streptococcus pneumoniae, Haemophilus influenzae, Niesseria gonorrhoeae and Fusobacterium nucleatum. The study also tried to compare how bacteria survived in Eswab which was refrigerated and in Eswab room temperature. Results for H. influenzae (n=9) and N. gonorrhoeae (n=9) showed that an equal amount or more of the bacteria survived in Eswab. More of F. nucleatum (n=9) survived in Copan swab (Copan swab sticks) for the first 28 hours, additionally they showed that the bacteria won’t survive more than 28 hours in room temperature. Regarding S. pneumoniae (n=9) and C. jejuni (n=9) both displayed unreliable results. Overall more measurements and additional studies are needed for more reliable results.

Place, publisher, year, edition, pages
2017. , 22 p.
Keyword [en]
Eswab, Haemophilus influenzae, Niesseria gonorrhoeae, Fusobacterium nucleatum, specimen transport
Keyword [sv]
Eswab, Haemophilus influenzae, Niesseria gonorrhoeae, Fusobacterium nucleatum, transportmedium
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:hj:diva-36170ISRN: JU-HHJ-BLA-1-20170054OAI: oai:DiVA.org:hj-36170DiVA: diva2:1110683
External cooperation
Länssjukhuset ryhov, laboratoriemedicin, mikrobiologen
Subject / course
HHJ, Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2017-07-05 Created: 2017-06-16 Last updated: 2017-07-05Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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