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Jämförelse mellan Grocotts manuella och automatiserade färgningsmetod
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2017 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Grocott metoden är den känsligaste metoden för infärgning av svampstrukturer i histologisk vävnad i jämförelse med till exempel Periodic Acid-Schiff (PAS). Handhavandet av det cancerogena oxidationsmedlet kromsyra samt tidsaspekten för Grocott är signifikanta problem. Syftet med studien var att undersöka om den automatiserade färgningsmetoden för Grocott ger likvärdiga resultat som vid den manuella färgningsmetoden. Tolv preparat färgades med både den manuella och den automatiserade metoden för Grocott. Den automatiserade färgningsmetoden använder ett färdigt kit med alla ingående lösningar. Visuell bedömning av preparaten utfördes med hjälp av legitimerade biomedicinska analytiker samt en patolog. Svampstrukturer i åtta av ela preparat samt kontroll bedömdes vara tydligare infärgade med den automatiserade metoden i färhållande till den manuella. Svampstrukturer i endast ett preparat bedömdes vara tydligare infärgade med den manuella metoden. Resterande två preparat visade inga svampstrukturer. Resultaten tyder på att den automatiserade färgningsmetoden för Grocott ger likvärdiga, alternativt tydligare infrägning av svampstrukturer i jämförelse med den manuella metoden. 

Abstract [en]

The Grocott method is the most sensitive staining method for fungal structures in histological tissues in comparison to for example Periodic Acid-Schiff (PAS). Usage of the cancerogenic chromic acid and the time aspect for Grocott are significant problems. The aim of this study was to examine if the automated staining method for Grocott provides equivalent results in comparison to the manual staining method. Twelve tissue sections were stained with both the automated and the manual method. The automated method contains a kit with all solutions. Visual evaluation was conducted with authorized biomedical scientists and a pathologist. Fungi structures in eight of eleven tissue sections and a control were evaluated as more distinct in the automated method in comparison to the manual method. Fungal structures in one of the tissue sections were evaluated more distinct in the manual method. In the remaining two tissue sections, no fungal structures were identified. Results from this study indicates that the automated staining method for Grocott provides equally, alternatively more distinct fungal staining in comparison to the manual method.

Place, publisher, year, edition, pages
2017. , 13 p.
Keyword [en]
Histology, Grocott, Fungi, Benchmark Special Stains, Chromic Acid, method comparison
Keyword [sv]
Histologi, Grocott, Svamp, Benchmark Special Stains, Kromsyra, metodjämförelse
National Category
Medical and Health Sciences Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-36077ISRN: JU-HHJ-BLA-1-20170048OAI: oai:DiVA.org:hj-36077DiVA: diva2:1109321
Subject / course
HHJ, Biomedical Laboratory Science
Presentation
2017-06-07, Länssjukhuset Ryhov, Jönköping, 22:35 (Swedish)
Supervisors
Examiners
Available from: 2017-06-27 Created: 2017-06-13 Last updated: 2017-06-27Bibliographically approved

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CiteExportLink to record
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