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Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion
Department of Cell and Molecular Biology, Microbiology, University of Gothenburg, Gothenburg, Sweden.
Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
Jönköping University, School of Engineering, JTH, Chemical Engineering.
Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
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2011 (English)In: Traffic: the International Journal of Intracellular Transport, ISSN 1398-9219, E-ISSN 1600-0854, Vol. 12, no 6, 740-753 p.Article in journal (Refereed) Published
Abstract [en]

We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.

Place, publisher, year, edition, pages
John Wiley & Sons, 2011. Vol. 12, no 6, 740-753 p.
Keyword [en]
Exocytosis, Golgi, ITRAQ, Membrane trafficking, Proteomics, Vesicles, cell protein, protein sro7p, unclassified drug, article, cell vacuole, Golgi complex, nonhuman, priority journal, protein analysis, protein content, protein secretion, protein targeting, quantitative analysis, tandem mass spectrometry, yeast, Adaptor Proteins, Signal Transducing, Biological Markers, Cytoplasmic Vesicles, Golgi Apparatus, Protein Transport, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:hj:diva-35592DOI: 10.1111/j.1600-0854.2011.01186.xISI: 000290407100009PubMedID: 21477180Scopus ID: 2-s2.0-79955709621OAI: oai:DiVA.org:hj-35592DiVA: diva2:1098229
Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2017-05-23Bibliographically approved

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