Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Localization of donor and acceptor sites of NADH dehydrogenase activities using inside-out and right-side-out plasma membrane vesicles from plants
Jönköping University, School of Education and Communication, HLK, Disciplinary Research. Department of Plant Physiology, University of Lund, Lund, Sweden.ORCID iD: 0000-0003-0117-2974
Department of Plant Physiology, University of Lund, Lund, Sweden.
Department of Plant Physiology, University of Lund, Lund, Sweden.
1988 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 239, no 1, p. 23-28Article in journal (Refereed) Published
Abstract [en]

Inside-out and right-side-out plasma membrane vesicles from sugar beet (Beta vulgaris L.) leaves, prepared by aqueous two-phase partitioning, were used to localize donor and acceptor sites and to determine substrate affinities for plasma membrane-bound NADH dehydrogenase activities. NADH-ferricyanide and NADH-cytochrome c reductase activities were approx. 30% latent with inside-out vesicles and about 80% latent with right-side-out vesicles, indicating that both donor and acceptor sites for these activities are located on the cytoplasmic surface of the plasma membrane, and that a possible transplasma membrane electron transport would constitute only a minor proportion of the total activity.

Place, publisher, year, edition, pages
1988. Vol. 239, no 1, p. 23-28
Keywords [en]
Plasma membrane; Inside-out vesicle; Redox chain; Enzyme kinetics; NADH-ferricyanide reductase; NADH-cytochrome c reductase
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:hj:diva-3869DOI: 10.1016/0014-5793(88)80538-6OAI: oai:DiVA.org:hj-3869DiVA, id: diva2:34689
Available from: 2007-10-17 Created: 2007-10-17 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

fulltext(1171 kB)198 downloads
File information
File name FULLTEXT01.pdfFile size 1171 kBChecksum SHA-512
10b159ec308b97538da9e24d1a15cb97dec68759a548064caac3b51d9ab4300ea28fd95f3007fbe178b605e8b1ac02dd475ac686c5e36143616479af5b044154
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Askerlund, Per

Search in DiVA

By author/editor
Askerlund, Per
By organisation
HLK, Disciplinary Research
In the same journal
FEBS Letters
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 198 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 458 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf