Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Calmodulin-stimulated Ca2+-ATPases in the vacuolar and plasma membranes in cauliflower
Högskolan i Jönköping, Högskolan för lärande och kommunikation, HLK, Ämnesforskning. Department of Plant Biochemistry, Lund University, Lund, Sweden.ORCID-id: 0000-0003-0117-2974
1997 (engelsk)Inngår i: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 114, nr 3, s. 999-1007Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The subcellular locations of Ca2+-ATPases in the membranes of cauliflower (Brassica oleracea L.) inflorescences were investigated. After continuous sucrose gradient centrifugation a 111-kD calmodulin (CaM)-stimulated and CaM-binding Ca2+-ATPase (BCA1; P. Askerlund [1996] Plant Physiol 110: 913–922; S. Malmstrom, P. Askerlund, M.G. Palmgren [1997] FEBS Lett 400: 324–328) comigrated with vacuolar membrane markers, whereas a 116-kD CaM-binding Ca2+-ATPase co-migrated with a marker for the plasma membrane. The 116-kD Ca2+-ATPase was enriched in plasma membranes obtained by aqueous two-phase partitioning, which is in agreement with a plasma membrane location of this Ca2+-ATPase. Countercurrent distribution of a low-density intracellular membrane fraction in an aqueous two-phase system resulted in the separation of the endoplasmic reticulum and vacuolar membranes. The 111-kD Ca2+-ATPase co-migrated with a vacuolar membrane marker after countercurrent distribution but not with markers for the endoplasmic reticulum. A vacuolar membrane location of the 111-kD Ca2+-ATPase was further supported by experiments with isolated vacuoles from cauliflower: (a) Immunoblotting with an antibody against the 111-kD Ca2+-ATPase showed that it was associated with the vacuoles, and (b) ATP-dependent Ca2+ uptake by the intact vacuoles was found to be CaM stimulated and partly protonophore insensitive.

sted, utgiver, år, opplag, sider
1997. Vol. 114, nr 3, s. 999-1007
Emneord [en]
Calcium-Transporting ATPases/isolation & purification/*metabolism, Calmodulin/*pharmacology, Cell Fractionation, Cell Membrane/enzymology/ultrastructure, Centrifugation; Density Gradient, Egtazic Acid/pharmacology, Vacuoles/*enzymology/ultrastructure, Vegetables/*enzymology
HSV kategori
Identifikatorer
URN: urn:nbn:se:hj:diva-2173PubMedID: 9232880OAI: oai:DiVA.org:hj-2173DiVA, id: diva2:32993
Tilgjengelig fra: 2007-10-17 Laget: 2007-10-17 Sist oppdatert: 2017-12-12bibliografisk kontrollert

Open Access i DiVA

fulltext(2614 kB)127 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2614 kBChecksum SHA-512
dba020ab6c2c63159ebb7bab5fa37b8f9660d1aba309c41d7d746dcf36a79fa492eefdcf1a7262b78211d363435fdcabeac67d71fa45042c6cfcc540c6aba132
Type fulltextMimetype application/pdf

PubMed

Personposter BETA

Askerlund, Per

Søk i DiVA

Av forfatter/redaktør
Askerlund, Per
Av organisasjonen
I samme tidsskrift
Plant Physiology

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 127 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

pubmed
urn-nbn

Altmetric

pubmed
urn-nbn
Totalt: 503 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf