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Metataxonomic and metaproteomic profiling of the oral microbiome in oral lichen planus: a pilot study
Department of Dental Medicine, Karolinska Institute, Huddinge, Sweden.
Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain.
Jönköping University, School of Health and Welfare, HHJ. Centre for Oral Health. Jönköping University, School of Health and Welfare, HHJ, Dept. of Natural Science and Biomedicine.ORCID iD: 0000-0002-9127-3877
Nanoxis Consulting AB, Gothenburg, Sweden.
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2023 (English)In: Journal of Oral Microbiology, ISSN 2000-2297, E-ISSN 2000-2297, Vol. 15, no 1, article id 2161726Article in journal (Refereed) Published
Abstract [en]

Background: A growing body of evidence demonstrates a different bacterial composition in the oral cavity of patients with oral lichen planus (OLP).

Patients and methods: Buccal swab samples were collected from affected and non-affected sites of six patients with reticular OLP and the healthy oral mucosa of six control subjects. 16S rRNA gene MiSeq sequencing and mass spectrometry-based proteomics were utilised to identify the metataxonomic and metaproteomic profiles of the oral microbiome in both groups.

Results: From the metataxonomic analysis, the most abundant species in the three subgroups were Streptococcus oralis and Pseudomonas aeruginosa, accounting for up to 70% of the total population. Principal Coordinates Analysis showed differential clustering of samples from the healthy and OLP groups. ANCOM-BC compositional analysis revealed multiple species (including P. aeruginosa and several species of Veillonella, Prevotella, Streptococcus and Neisseria) significantly over-represented in the control group and several (including Granulicatella elegans, Gemella haemolysans and G. parahaemolysans) in patients with OLP. The metaproteomic data were generally congruent and revealed that several Gemella haemolysans-belonging peptidases and other proteins with inflammatory and virulence potential were present in OLP lesions.

Conclusion: Our data suggest that several bacterial species are associated with OLP. Future studies with larger cohorts should be conducted to determine their role in the aetiology of OLP and evaluate their potential as disease biomarkers.

Place, publisher, year, edition, pages
Taylor & Francis, 2023. Vol. 15, no 1, article id 2161726
Keywords [en]
metaproteomics, metataxonomics, oral bacteria, Oral cavity, oral lichen planus, oral microbiome, oral mucosa
National Category
Dentistry
Identifiers
URN: urn:nbn:se:hj:diva-59342DOI: 10.1080/20002297.2022.2161726ISI: 000905311100001PubMedID: 36605405Scopus ID: 2-s2.0-85145288158Local ID: GOA;intsam;852236OAI: oai:DiVA.org:hj-59342DiVA, id: diva2:1724878
Available from: 2023-01-09 Created: 2023-01-09 Last updated: 2023-01-27Bibliographically approved

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Mira, Alex

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