An ecosystem approach to innovation and business has become increasingly relevant in contemporary research but research knowledge is scattered across divergent disciplines. The aim of this study is thus, on the basis of an extensive, multidisciplinary literature review to integrate the extant knowledge on innovation and business ecosystems and analyze how they are conceptualized, analyzed, captured and depicted. By conducting a systematic multi-phase content analysis of over 230 articles selected from the Web of Science, we will build a comprehensive picture on the research streams of innovation/business ecosystem research, the used methods, foci, illustrations/visualizations of business/innovation ecosystems and build a research agenda for future research. This article contributes by providing a structured analysis on this multi-disciplinary research area, aggregating the current knowledge and generating a research agenda on innovation/business ecosystems - a theme that is emergent, multifaceted, and crucial to innovative companies as well as researchers in the fields of innovation, management, technology and marketing.