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Tompa, Andrea
Publications (8 of 8) Show all publications
Tompa, A., Åkesson, K., Karlsson, S. & Faresjö, M. (2019). Suppressed immune profile in children with type 1 diabetes in combination with celiac disease. In: : . Paper presented at ESCCA 2019, Flowrescence in the Fjords, Bergen, Norway, 18-21 September 2019.
Open this publication in new window or tab >>Suppressed immune profile in children with type 1 diabetes in combination with celiac disease
2019 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

Introduction: Cytokines, chemokines, acute phase proteins (APP), adipocytokines and matrix metalloproteinases (MMP) are involved in different pathophysiological processes of inflammatory character. The role of the different immune markers and the peripheral immunoregulatory milieu in children diagnosed with type 1 diabetes (T1D) in combination with celiac disease (CD) is not fully understood and is not well studied. The purpose of the present study was therefore to acquire more knowledge and to gain deeper understanding on peripheral immunoregulatory milieu in children with T1D and/or CD.

Methods: The study included children diagnosed with T1D in combination with CD (n=18), children with T1D (n=27) or CD (n=16), and reference children (n=42).

Blood samples were collected, and serum stored in -80°C until analysis, avoiding multiple freeze-thaw cycles. The inflammatory cyto/chemokines (IL-1β, -5, -6, -8, -9, -10, -13, -15, -17A, -22, -25, -33, IFN-γ, TNF-α, G-CSF, MCP-1, MIP-1α, MIP-1β), diabetes related immune markers (visfatin, resistin), APP (procalcitonin (PTC), ferritin, tissue protein activator, fibrinogen, serum amyloid A) and matrix metalloproteinases (MMP-1, -2, -3) were analyzed with Luminex technique using Bio-Plex assays. Hierarchical cluster analysis was used to identify similarities/differences in immune profiles between children with double diagnosis and children with single diagnosis and reference children. Mann-Whitney U test was used for comparison of the different diagnosis groups within the clusters and whole cohort, respectively.

Results: The largest cluster included 75% of the participants and the diagnose distribution in the cluster were very similar to the distribution in the whole study cohort. The remaining 25% were divided in two smaller clusters representing 15.5% and 6.5% respectively. The major finding of this study showed that children with double diagnosis had (1) lower serum levels of IL-22, MCP-1, PCT, visfatin and MMP-2 compared to children with T1D; and (2) lower serum levels of the APC associated chemokine MIP-1α compared to reference children, observed in the main cluster. Most of these observations were also seen in the whole cohort.  

Conclusion: Our observations indicate decreased serum levels of IL-22, MIP-1α, MCP-1, PCT, visfatin and MMP-2 in children diagnosed with T1D in combination with CD. These results indicate a suppressed immune profile including Th17 cytokines, chemokines, acute phase proteins, diabetes-related and matrix metalloproteinase immune markers. Functional studies of the involved immune cells (CD4+ Treg, CD8+ Treg, NK-cells and dendritic cells) could contribute to elucidate the heterogeneous immunological processes in children with more than one autoimmune disease.

National Category
Pediatrics Immunology in the medical area
Identifiers
urn:nbn:se:hj:diva-47397 (URN)
Conference
ESCCA 2019, Flowrescence in the Fjords, Bergen, Norway, 18-21 September 2019
Available from: 2020-01-14 Created: 2020-01-14 Last updated: 2020-01-14Bibliographically approved
Tompa, A. (2018). Subsets of CD4+, CD8+ and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation. In: : . Paper presented at ESCCA 2018 Conference: At the Shore of Future Cytometry, September 13–15, 2018, Valencia, Spain.
Open this publication in new window or tab >>Subsets of CD4+, CD8+ and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation
2018 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-42806 (URN)
Conference
ESCCA 2018 Conference: At the Shore of Future Cytometry, September 13–15, 2018, Valencia, Spain
Available from: 2019-02-01 Created: 2019-02-01 Last updated: 2019-02-01Bibliographically approved
Tompa, A., Nilsson-Bowers, A. & Faresjö, M. (2018). Subsets of CD4+, CD8+, and CD25hi Lymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation. Journal of Immunology, 201(6), 1799-1809
Open this publication in new window or tab >>Subsets of CD4+, CD8+, and CD25hi Lymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation
2018 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 201, no 6, p. 1799-1809Article in journal (Refereed) Published
Abstract [en]

Several key factors can affect the outcome of immunological studies; isolation/cryopreservation can possibly alter T, B, NK, and T-regulatory (Treg) cell marker expression patterns. Blood samples from 50 blood donors supplemented with Na-heparin or K2EDTA were handled within 4 and 24 h after blood sampling. PBMC were isolated with different density gradients. Flow cytometric analysis of intracellular and extracellular CD markers was performed on blood samples freshly isolated PBMC, and PBMC was thawed 6 and 12 mo post-cryopreservation for the purpose of identifying B, NK, Th, T-cytotoxic, and Treg cells. No differences were observed in the percentages for CD3+, CD3+CD4+, CD3+CD8+, CD19+, or CD56+CD16+ cells within 24 h of sampling regardless of which supplement or isolation techniques were used. Differentiated (diff) CD4+ cells were in general less affected by isolation and cryopreservation than diff CD8+ cells. Terminally diff effector CD4+ and CD8+ cells were not affected by either isolation of lymphocytes or cryopreservation. In contrast, naive and early-diff effector memory CD4+ and CD8+ cells were affected by isolation and cryopreservation. The percentages of Treg cells defined as CD4+CD25hi expressing CD101 or CD129, CD4+CD25hiCD127, and CD4+CD25hiCD127FOXP3+, respectively, remained stable after isolation and cryopreservation. Subsets expressing CD127, with or without FOXP3, were not affected by isolation/cryopreservation. Subsets expressing CD39, contrary to CD45RA, on CD4+CD25+CD127 cells with or without FOXP3 were not affected by either isolation or cryopreservation. In conclusion, subsets of CD4+, CD8+, and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation.

Place, publisher, year, edition, pages
American Association of Immunologists, 2018
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-41150 (URN)10.4049/jimmunol.1701409 (DOI)000443585800020 ()30082322 (PubMedID)2-s2.0-85053143384 (Scopus ID)
Funder
Futurum - Academy for Health and Care, Jönköping County Council, Sweden
Available from: 2018-08-15 Created: 2018-08-15 Last updated: 2018-09-25Bibliographically approved
Carlsson, E., Magnusson, A., Tompa, A., Bülow, P., Gerdner, A. & Faresjö, M. (2016). Psychological stress affects the numbers of circulating CD56+CD16+ and CD4+CD25+FoxP3+CD127- cells and induce an immune response towards type 1 diabetes-related autoantigens in young women.
Open this publication in new window or tab >>Psychological stress affects the numbers of circulating CD56+CD16+ and CD4+CD25+FoxP3+CD127- cells and induce an immune response towards type 1 diabetes-related autoantigens in young women
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2016 (English)Article in journal (Other academic) Submitted
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-31751 (URN)
External cooperation:
Available from: 2016-09-12 Created: 2016-09-12 Last updated: 2016-09-12
Cherfan, P., Tompa, A., Wikby, A., Löfgren, S. & Jonasson, L. (2007). Effects of simvastatin on human T cells in vivo. Atherosclerosis, 193(1), 186-192
Open this publication in new window or tab >>Effects of simvastatin on human T cells in vivo
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2007 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 193, no 1, p. 186-192Article in journal (Refereed) Published
Keywords
Adult, Apolipoproteins/blood, C-Reactive Protein/metabolism, Concanavalin A/pharmacology, Double-Blind Method, Enterotoxins/pharmacology, HLA-DR Antigens/blood, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors/*pharmacology, Hypercholesterolemia/blood/drug therapy/immunology, Interleukin-2 Receptor alpha Subunit/blood, L-Selectin/blood, Lipids/blood, Male, Middle Aged, Simvastatin/*pharmacology, T-Lymphocyte Subsets/drug effects, T-Lymphocytes/*drug effects/immunology/metabolism
Identifiers
urn:nbn:se:hj:diva-4293 (URN)16860807 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Nijm, J., Wikby, A., Tompa, A., Olsson, A. G. & Jonasson, L. (2005). Circulating levels of proinflammatory cytokines and neutrophil-platelet aggregates in patients with coronary artery disease.. American Journal of Cardiology, 95(4), 452-456
Open this publication in new window or tab >>Circulating levels of proinflammatory cytokines and neutrophil-platelet aggregates in patients with coronary artery disease.
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2005 (English)In: American Journal of Cardiology, ISSN 0002-9149, E-ISSN 1879-1913, Vol. 95, no 4, p. 452-456Article in journal (Refereed) Published
Keywords
Angina Pectoris/immunology/metabolism, Angina; Unstable/immunology/metabolism, C-Reactive Protein/*analysis, Case-Control Studies, Cholesterol; HDL/blood, Coronary Arteriosclerosis/*immunology/metabolism, Flow Cytometry, Humans, Interleukin 1 Receptor Antagonist Protein, Interleukins/*blood, Male, Middle Aged, Myocardial Infarction/immunology/metabolism, Neutrophils/*metabolism, Platelet Aggregation/*physiology, Receptors; Interleukin-1/antagonists & inhibitors, Receptors; Interleukin-2/blood, Recombinant Proteins/blood, Sialoglycoproteins/blood, T-Lymphocytes; Helper-Inducer/metabolism, Triglycerides/blood
Identifiers
urn:nbn:se:hj:diva-4300 (URN)15695127 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Jonasson, L., Tompa, A. & Wikby, A. (2003). Expansion of peripheral CD8+ T cells in patients with coronary artery disease: relation to cytomegalovirus infection.. Journal of Internal Medicine, 254(5), 472-478
Open this publication in new window or tab >>Expansion of peripheral CD8+ T cells in patients with coronary artery disease: relation to cytomegalovirus infection.
2003 (English)In: Journal of Internal Medicine, ISSN 0954-6820, E-ISSN 1365-2796, Vol. 254, no 5, p. 472-478Article in journal (Refereed) Published
Keywords
Adult, Antigens; CD28/blood, Antigens; CD57/blood, CD8-Positive T-Lymphocytes/*immunology, Coronary Arteriosclerosis/complications/*immunology, Cross-Sectional Studies, Cytomegalovirus Infections/complications/*immunology, Humans, Immunity; Cellular, Immunophenotyping, Inflammation Mediators/blood, Lymphocyte Activation, Lymphocyte Count, Male, Middle Aged, T-Lymphocyte Subsets/*immunology
Identifiers
urn:nbn:se:hj:diva-4303 (URN)14535969 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Hyland, P., Duggan, O., Turbitt, J., Coulter, J., Wikby, A., Johansson, B., . . . Barnett, Y. (2002). Nonagenarians from the Swedish NONA Immune Study have increased plasma antioxidant capacity and similar levels of DNA damage in peripheral blood mononuclear cells compared to younger control subjects. Experimental Gerontology, 37(2-3), 465-473
Open this publication in new window or tab >>Nonagenarians from the Swedish NONA Immune Study have increased plasma antioxidant capacity and similar levels of DNA damage in peripheral blood mononuclear cells compared to younger control subjects
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2002 (English)In: Experimental Gerontology, ISSN 0531-5565, E-ISSN 1873-6815, Vol. 37, no 2-3, p. 465-473Article in journal (Refereed) Published
Keywords
Adult, Aged, Aged; 80 and over, Aging/blood/*genetics/immunology/*metabolism, Antioxidants/*metabolism, Comparative Study, DNA Damage, Female, Ferric Compounds/metabolism, Ferrous Compounds/metabolism, Humans, Leukocytes; Mononuclear, Male, Middle Aged, Oxidation-Reduction, Research Support; Non-U.S. Gov't, Sweden
Identifiers
urn:nbn:se:hj:diva-5219 (URN)11772534 (PubMedID)
Available from: 2008-07-08 Created: 2008-07-08 Last updated: 2017-12-12Bibliographically approved
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