Change search
Link to record
Permanent link

Direct link
BETA
Tompa, Andrea
Publications (7 of 7) Show all publications
Tompa, A. (2018). Subsets of CD4+, CD8+ and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation. In: : . Paper presented at ESCCA 2018 Conference: At the Shore of Future Cytometry, September 13–15, 2018, Valencia, Spain.
Open this publication in new window or tab >>Subsets of CD4+, CD8+ and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation
2018 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-42806 (URN)
Conference
ESCCA 2018 Conference: At the Shore of Future Cytometry, September 13–15, 2018, Valencia, Spain
Available from: 2019-02-01 Created: 2019-02-01 Last updated: 2019-02-01Bibliographically approved
Tompa, A., Nilsson-Bowers, A. & Faresjö, M. (2018). Subsets of CD4+, CD8+, and CD25hi Lymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation. Journal of Immunology, 201(6), 1799-1809
Open this publication in new window or tab >>Subsets of CD4+, CD8+, and CD25hi Lymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation
2018 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 201, no 6, p. 1799-1809Article in journal (Refereed) Published
Abstract [en]

Several key factors can affect the outcome of immunological studies; isolation/cryopreservation can possibly alter T, B, NK, and T-regulatory (Treg) cell marker expression patterns. Blood samples from 50 blood donors supplemented with Na-heparin or K2EDTA were handled within 4 and 24 h after blood sampling. PBMC were isolated with different density gradients. Flow cytometric analysis of intracellular and extracellular CD markers was performed on blood samples freshly isolated PBMC, and PBMC was thawed 6 and 12 mo post-cryopreservation for the purpose of identifying B, NK, Th, T-cytotoxic, and Treg cells. No differences were observed in the percentages for CD3+, CD3+CD4+, CD3+CD8+, CD19+, or CD56+CD16+ cells within 24 h of sampling regardless of which supplement or isolation techniques were used. Differentiated (diff) CD4+ cells were in general less affected by isolation and cryopreservation than diff CD8+ cells. Terminally diff effector CD4+ and CD8+ cells were not affected by either isolation of lymphocytes or cryopreservation. In contrast, naive and early-diff effector memory CD4+ and CD8+ cells were affected by isolation and cryopreservation. The percentages of Treg cells defined as CD4+CD25hi expressing CD101 or CD129, CD4+CD25hiCD127, and CD4+CD25hiCD127FOXP3+, respectively, remained stable after isolation and cryopreservation. Subsets expressing CD127, with or without FOXP3, were not affected by isolation/cryopreservation. Subsets expressing CD39, contrary to CD45RA, on CD4+CD25+CD127 cells with or without FOXP3 were not affected by either isolation or cryopreservation. In conclusion, subsets of CD4+, CD8+, and CD25hi lymphocytes are in general not influenced by isolation and long-term cryopreservation.

Place, publisher, year, edition, pages
American Association of Immunologists, 2018
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-41150 (URN)10.4049/jimmunol.1701409 (DOI)000443585800020 ()30082322 (PubMedID)2-s2.0-85053143384 (Scopus ID)
Funder
Futurum - Academy for Health and Care, Jönköping County Council, Sweden
Available from: 2018-08-15 Created: 2018-08-15 Last updated: 2018-09-25Bibliographically approved
Carlsson, E., Magnusson, A., Tompa, A., Bülow, P., Gerdner, A. & Faresjö, M. (2016). Psychological stress affects the numbers of circulating CD56+CD16+ and CD4+CD25+FoxP3+CD127- cells and induce an immune response towards type 1 diabetes-related autoantigens in young women.
Open this publication in new window or tab >>Psychological stress affects the numbers of circulating CD56+CD16+ and CD4+CD25+FoxP3+CD127- cells and induce an immune response towards type 1 diabetes-related autoantigens in young women
Show others...
2016 (English)Article in journal (Other academic) Submitted
National Category
Immunology
Identifiers
urn:nbn:se:hj:diva-31751 (URN)
External cooperation:
Available from: 2016-09-12 Created: 2016-09-12 Last updated: 2016-09-12
Cherfan, P., Tompa, A., Wikby, A., Löfgren, S. & Jonasson, L. (2007). Effects of simvastatin on human T cells in vivo. Atherosclerosis, 193(1), 186-192
Open this publication in new window or tab >>Effects of simvastatin on human T cells in vivo
Show others...
2007 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 193, no 1, p. 186-192Article in journal (Refereed) Published
Keywords
Adult, Apolipoproteins/blood, C-Reactive Protein/metabolism, Concanavalin A/pharmacology, Double-Blind Method, Enterotoxins/pharmacology, HLA-DR Antigens/blood, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors/*pharmacology, Hypercholesterolemia/blood/drug therapy/immunology, Interleukin-2 Receptor alpha Subunit/blood, L-Selectin/blood, Lipids/blood, Male, Middle Aged, Simvastatin/*pharmacology, T-Lymphocyte Subsets/drug effects, T-Lymphocytes/*drug effects/immunology/metabolism
Identifiers
urn:nbn:se:hj:diva-4293 (URN)16860807 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Nijm, J., Wikby, A., Tompa, A., Olsson, A. G. & Jonasson, L. (2005). Circulating levels of proinflammatory cytokines and neutrophil-platelet aggregates in patients with coronary artery disease.. American Journal of Cardiology, 95(4), 452-456
Open this publication in new window or tab >>Circulating levels of proinflammatory cytokines and neutrophil-platelet aggregates in patients with coronary artery disease.
Show others...
2005 (English)In: American Journal of Cardiology, ISSN 0002-9149, E-ISSN 1879-1913, Vol. 95, no 4, p. 452-456Article in journal (Refereed) Published
Keywords
Angina Pectoris/immunology/metabolism, Angina; Unstable/immunology/metabolism, C-Reactive Protein/*analysis, Case-Control Studies, Cholesterol; HDL/blood, Coronary Arteriosclerosis/*immunology/metabolism, Flow Cytometry, Humans, Interleukin 1 Receptor Antagonist Protein, Interleukins/*blood, Male, Middle Aged, Myocardial Infarction/immunology/metabolism, Neutrophils/*metabolism, Platelet Aggregation/*physiology, Receptors; Interleukin-1/antagonists & inhibitors, Receptors; Interleukin-2/blood, Recombinant Proteins/blood, Sialoglycoproteins/blood, T-Lymphocytes; Helper-Inducer/metabolism, Triglycerides/blood
Identifiers
urn:nbn:se:hj:diva-4300 (URN)15695127 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Jonasson, L., Tompa, A. & Wikby, A. (2003). Expansion of peripheral CD8+ T cells in patients with coronary artery disease: relation to cytomegalovirus infection.. Journal of Internal Medicine, 254(5), 472-478
Open this publication in new window or tab >>Expansion of peripheral CD8+ T cells in patients with coronary artery disease: relation to cytomegalovirus infection.
2003 (English)In: Journal of Internal Medicine, ISSN 0954-6820, E-ISSN 1365-2796, Vol. 254, no 5, p. 472-478Article in journal (Refereed) Published
Keywords
Adult, Antigens; CD28/blood, Antigens; CD57/blood, CD8-Positive T-Lymphocytes/*immunology, Coronary Arteriosclerosis/complications/*immunology, Cross-Sectional Studies, Cytomegalovirus Infections/complications/*immunology, Humans, Immunity; Cellular, Immunophenotyping, Inflammation Mediators/blood, Lymphocyte Activation, Lymphocyte Count, Male, Middle Aged, T-Lymphocyte Subsets/*immunology
Identifiers
urn:nbn:se:hj:diva-4303 (URN)14535969 (PubMedID)
Available from: 2007-10-31 Created: 2007-10-31 Last updated: 2017-12-12Bibliographically approved
Hyland, P., Duggan, O., Turbitt, J., Coulter, J., Wikby, A., Johansson, B., . . . Barnett, Y. (2002). Nonagenarians from the Swedish NONA Immune Study have increased plasma antioxidant capacity and similar levels of DNA damage in peripheral blood mononuclear cells compared to younger control subjects. Experimental Gerontology, 37(2-3), 465-473
Open this publication in new window or tab >>Nonagenarians from the Swedish NONA Immune Study have increased plasma antioxidant capacity and similar levels of DNA damage in peripheral blood mononuclear cells compared to younger control subjects
Show others...
2002 (English)In: Experimental Gerontology, ISSN 0531-5565, E-ISSN 1873-6815, Vol. 37, no 2-3, p. 465-473Article in journal (Refereed) Published
Keywords
Adult, Aged, Aged; 80 and over, Aging/blood/*genetics/immunology/*metabolism, Antioxidants/*metabolism, Comparative Study, DNA Damage, Female, Ferric Compounds/metabolism, Ferrous Compounds/metabolism, Humans, Leukocytes; Mononuclear, Male, Middle Aged, Oxidation-Reduction, Research Support; Non-U.S. Gov't, Sweden
Identifiers
urn:nbn:se:hj:diva-5219 (URN)11772534 (PubMedID)
Available from: 2008-07-08 Created: 2008-07-08 Last updated: 2017-12-12Bibliographically approved
Organisations

Search in DiVA

Show all publications